五苓散对高脂血症模型贵州小型猪的治疗作用

Therapeutic Effect of Wuling Powder on Guizhou Mini-pigs of Hyperlipidemia Model

目的研究五苓散对高脂饮食诱导的高脂血症模型贵州小型猪的治疗作用。方法取12只健康贵州小型猪,其中4只为正常组,饲喂普通饲料;另8只饲喂高脂饲料,连续2 4周,建立高脂血症模型。造模成功后,分为模型组和五苓散治疗组,均继续喂高脂饲料。治疗组饲料中每日加入五苓散0.5 5 g/k g。连续给药8周后,监测各组体质量、体尺、腹围、血脂和主要中医证候体征变化。结果造模完成后,模型组小型猪较正常组体质量指数和腹围均值有所增加,但差异不显著(均P>0.0 5);模型组主要血脂指标血清总胆固醇(T C)、三酰甘油(T G)和低密度脂蛋白胆固醇(LDL-C)明显升高(均P<0.05),血清高密度脂蛋白胆固醇(HDL-C)明显降低(P<0.0 5);模型组动物表现出肥胖臃肿、腹部膨大、被毛稀少脱落、神疲乏力、好静懒动和粪便色淡稀软不成形等高脂血症表征。经五苓散干预后,治疗组较模型组的体质量指数和腹围有所下降(P>0.05),中医证候综合评分明显降低,主要血脂指标TC、TG、LDL-C明显降低(均P<0.05),HDL-C明显升高(P<0.05)。结论成功构建了高脂血症贵州小型猪模型,五苓散干预能明显改善高脂血症模型的血脂代谢紊乱情况。

Objective To study the therapeutic effect of Wuling powder on hyperlipidemia induced by a high-fat diet in Guizhou mini-pigs. Methods Twelve healthy Guizhou mini-pigs were selected, 4 pigs fed a standard diet(normal group), and 8 pigs fed a high-fat diet for 24 weeks to establish a hyperlipidemia model.Further, the model pigs were divided into model group and Wuling powder treatment group, and fed a high-fat diet for 8 weeks. Wuling powder(0.55 g/kg) was added to the diet of the treatment group every day.Body weight, body size, abdominal girth, serum lipid parameters, and main syndromes and signs were measured and recorded. Results After the animal model was established successfully, body weight and body mass index(BMI) of the model group were found to be higher than that of the normal group, although the difference was not significant(P>0.05). The levels of the following main blood lipid parameters:total cholesterol(TC), triglyceride(TG), and low-density lipoprotein cholesterol(LDL-C) increased significantly(all P<0.05) in the model group, but high-density lipoprotein cholesterol(HDL-C) level decreased significantly(P<0.05) as compared with the normal group. The pigs in the model group showed obesity, abdominal enlargement, hair thinning, tiredness and fatigue, and light or soft stool, which are syndroms of hyperlipidemia. After Wuling powder treatment, body weight and BMI of the mini-pigs in the treatment group were lower than those in the model group(P>0.05);the comprehensive score of traditional Chinese medicine syndrome was significantly reduced, in addition to the TC, TG, and LDL-C levels(P<0.05);HDL-C levels were significantly increased(P<0.05). Conclusion The hyperlipidemia model is successfully established in Guizhou mini-pigs, and lipid metabolism disorder can be improved to a certain degree after intervention treatment with Wuling powder.