新型材料固定化氧化还原酶研究进展

Advance of new materials for the immobilization of oxidoreductases

氧化还原酶可催化许多关键反应,在生物制造中具有重要应用价值.本文关注新型材料在氧化还原酶固定化中的应用和研究进展,总结碳材料、导电高分子、金属-有机框架材料等以及复合材料固定化酶的研究现状,分析各种材料的介观结构、理化性能,及其在酶固定中的优势和缺点.探讨以提高材料生物相容性从而提升氧化还原酶稳定性和催化效率为导向的界面修饰调控策略.着重介绍导电材料固定化氧化还原酶,及其在生物转化、生物传感器、生物燃料电池等领域的应用.展望氧化还原酶固定化的发展趋势和所面临的挑战.

Oxidoreductases catalyze a myriad of important reactions in biomanufacturing.Currently,various novel materials for oxidoreductases immobilization had been developed.Recent research based on carbon materials,conductive polymer,metal organic framework,and hybrids materials were reviewed.Chemical and physical characteristics of carriers with advantages and disadvantages were summarized.Strategies to improve the biocompatibility of carrier by surface modification and promote activity and stability of enzyme were addressed.Conductive materials for oxidoreductases immobilization and their application in bioelectrocatalysis,biosensor,and biofuel cells were discussed.Furthermore,challenges and perspectives in oxidoreductases immobilization were highlighted.