猪精子核酸识别Toll样受体的表达特性

Expression and Distribution of Nucleic Acid-sensing TLRs in Porcine Sperm

【目的】Toll样受体(Toll like receptors,TLRs)作为天然免疫系统中重要的模式识别受体,在抵御病原微生物感染方面发挥着重要作用。研究猪精子核酸识别TLR的表达和分布情况,为精子TLR信号通路相关功能和抗微生物特性的研究奠定基础。【方法】采用RT-PCR技术检测公猪精子TLR3、7、8和9 mRNA的表达情况,利用免疫荧光方法检测了TLR3蛋白在猪精子上的分布。【结果】RT-PCR结果显示,TLR3、7、8和9 mRNA在猪精子均有表达;免疫荧光试验结果显示TLR3主要分布在精子头部的后部和中段。【结论】猪精子上核酸识别TLRs的存在以及TLR3分布的区域化提示猪精子可能对病毒及其衍生物感染做出应答,为进一步研究精子TLR信号通路提供依据。

【Objective】Expression and distribution of the toll-like receptor(TLR)in porcine sperm were analyzed to facilitate the study on the related signaling pathway and antimicrobial properties.【Method】The mRNA expressions of TLR3,7,8,and 9 in pig sperm were detected by RT-PCR,and the distribution of TLR3 by immunofluorescence.【Result】The mRNA of TLR3,7,8,and 9 were all expressed in the sperm,and TLR3 mainly found in the posterior head and midpiece.【Conclusion】The expression of nucleic acid-sensing TLRs and the localizations of TLR3 in the porcine sperm revealed in this study gave clues of how the sperm might respond to attacks by virus and derivatives.The information paved the way for further investigation on the sperm TLR signaling pathway and pathogen recognition function.