期刊文献+

乳铁蛋白基姜黄素纳米载体颗粒的制备及其对大鼠抗疲劳能力的影响 被引量:1

Preparation of Lactoferrin-Curcumin Nanoparticles and Its Effect on Antifatigue Ability in Rats
下载PDF
导出
摘要 本实验制备了乳铁蛋白基姜黄素纳米载体颗粒并对其结构进行了分析,通过体内吸收实验比对了姜黄素纳米载体颗粒与姜黄素单体在体内吸收的特性,在运动性疲劳大鼠模型下通过测定力竭游泳时长、血乳酸动态变化、肌糖原、肝糖原、血尿素氮含量以及抗氧化物酶活性和脂质过氧化产物在机体内的水平,综合评判姜黄素纳米载体颗粒的抗疲劳活性。结果显示,姜黄素纳米载体颗粒粒径约593.8 nm,在溶液中分布均一、形态稳定,具有较好的负载率(152.03±2.43)mg/g与包封率63.57%±0.74%。在体内吸收实验中,姜黄素纳米载体颗粒有效降低首过效应,能够在血浆中以较高浓度维持更长时间,表明乳铁蛋白载体起到了一定的缓释作用;在运动性疲劳模型中,大鼠在饲喂姜黄素纳米载体颗粒后,促进了肌糖原与肝糖原的合成,并能够显著提高力竭游泳的时长(P<0.05),大幅加速运动后90 min内血乳酸的代谢速率,并通过血尿素氮含量的降低、抗氧化物酶活性的提升以及脂质过氧化产物的下降。综合验证了姜黄素纳米载体颗粒具有抗疲劳活性,其抗疲劳活性与摄入剂量相关。 In this experiment,nanoparticles were prepared with lactoferrin and curcumin and their structures were analyzed.The absorption characteristics of curcumin nanoparticles and curcumin monomers were compared by in vivo absorption experiments.Then,the anti-fatigue activity of curcumin nanoparticles was evaluated by measuring the duration of exhaustive swimming time,dynamic changes of blood lactic acid,muscle glycogen content,liver glycogen content,blood urea nitrogen content,antioxidant enzyme activity and lipid peroxidation products in Rats.The results showed that the particle size of curcumin nanoparticles was about 593.8 nm.The distribution of curcumin nanoparticles was uniform and the morphology was stable.It has good loading rate of(152.03±2.43)mg/g and encapsulation efficiency rate of 63.57%±0.74%.Compared with curcumin nanoparticles,the first pass effect of curcumin nanoparticles in vivo was reduced,and it could be maintained at a higher concentration for a longer time in plasma,indicating that lactoferrin carrier played a certain role in sustained-release.In the exercise-induced fatigue animal model,the synthesis of muscle glycogen and liver glycogen were promoted after feeding curcumin nanoparticles,and the duration of exhaustive swimming was significantly increased(P<0.05).The metabolic rate of blood lactic acid was greatly accelerated within 90 minutes after exercise.The index of decreased BUM,MDA and increased GSH-PX also showed an improved anti-fatigue activity by feeding curcumin nanoparticles which was positively correlated with the dose.
作者 贾前生 刘远洋 JIA Qiansheng;LIU Yuanyang(Physical education and Research Department of Heilongjiang Bayi Agricultural University,Daqing 163000,China;Food College of Heilongjiang Bayi Agricultural University,Daqing 163000,China)
出处 《食品工业科技》 CAS 北大核心 2021年第13期26-32,共7页 Science and Technology of Food Industry
基金 黑龙江省高等教育教学改革研究计划项目(SJGZ20200121) 黑龙江省哲学社会科学研究计划资助(14D039) 黑龙江八一农垦大学校内培育基金资助项目(XRW2017-15)。
关键词 姜黄素 乳铁蛋白 纳米载体 运动性疲劳 curcumin lactoferrin nano carrier exercise-induced fatigue
  • 相关文献

参考文献13

二级参考文献139

共引文献143

同被引文献16

引证文献1

二级引证文献4

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部