白介素38在变应性鼻炎发病机制中作用的初步研究

Preliminary study on the role of interleukin 38 in the pathogenesis of allergic rhinitis

目的探讨白介素38(IL-38)与变应性鼻炎(AR)发病的关系及其机制。方法选取35例AR伴鼻中隔偏曲的患者和20例单纯鼻中隔偏曲的患者作为AR组和对照组,采集其鼻甲黏膜及外周血液,实时定量PCR法检测局部组织中IL-38mRNA的表达、免疫组化法检测局部组织内IL-38的表达;酶联免疫法(ELISA法)检测外周血中IL-38及Th17相关因子IL-17、IL-6的表达,同时以流式细胞术检测外周血中Th17细胞的表达,分析IL-38的表达与Th17相关指标水平的相关性;最后以重组IL-38干预AR患者外周血单个核细胞(PBMC)后检测Th17细胞的百分比率。结果AR组局部组织及外周血中的IL-38表达水平较对照组显著降低,Th17细胞水平、IL-17及IL-6表达水平显著升高,差异均有统计学意义(P<0.05)。AR组IL-38表达与Th17细胞水平、IL-17及IL-6呈显著负相关(P<0.05);重组IL-38干预后AR组PBMC中Th17细胞百分比率显著下降(P<0.05)。结论IL-38在AR的发生发展中有重要作用,在AR中的低表达引起其对Th17细胞的抑制作用减弱与AR的发病密切相关。

To investigate the relationship between interleukin-38(IL-38)and the pathogenesis of allergic rhinitis(AR),total of 35 patients with AR and 20 patients with simple deviation of nasal septum were employed as AR group and control group.The turbinate mucosa and peripheral blood were collected.The expression of IL-38 in local tissues was detected by immunohistochemistry,while the IL-38 mRNA expression in local tissues was detected by real-time quantitative PCR.Enzyme-linked immunosorbent assay(ELISA)was used to detect the expression of IL-38 and Th17 related factors IL-17 and IL-6 in peripheral blood.The expression of Th17 cells in peripheral blood was detected by flow cytometry;the correlation between the expression of IL-38 and Th17 related factors were analyzed.After the intervention with recombinant IL-38,the peripheral blood mononuclear cells(PBMC)of AR patients were subjected for the frequency of Th17 cells.Data showed that the expression level of IL-38 in the local tissues and peripheral blood of AR group was significantly lower than that of the control group,while the levels of Th17 cells,IL-17 and IL-6 were significantly higher than those of the control group(P<0.05).The expression of IL-38 in AR group was negatively correlated with Th17 cell level,IL-17 and IL-6(P<0.05).After intervention,the frequency of Th17 cells in PBMC of AR group decreased significantly(P<0.05).In conclusion,IL-38 plays an important role in the genesis and development of AR,and the low expression of IL-38 in AR leads to the decrease of its inhibition on Th17 cells,which is closely related to the pathogenesis of AR.