半夏野生品和栽培品中8种核苷类成分的含量测定方法建立及差异分析

Method Establishment for Content Determination of 8 Nucleosides in Wild and Cultivated Pinellia ternata and the Difference Analysis

目的:建立半夏野生品和栽培品中8种核苷类成分含量测定的方法,并进行两者的差异分析。方法:采用高效液相色谱法测定20批半夏药材(野生品YS1~YS8、栽培品ZP1~ZP12)中尿嘧啶、尿苷、肌苷、黄嘌呤、腺嘌呤、鸟苷、β-胸苷和腺苷的含量;以上述8种核苷类成分含量为基础,采用聚类分析、主成分分析(PCA)、偏最小二乘法-判别分析(OPLS-DA)等化学模式识别法对半夏野生品和栽培品进行综合评价。结果:20批半夏药材中尿嘧啶、黄嘌呤、尿苷、肌苷、腺嘌呤、鸟苷、β-胸苷和腺苷的含量分别为0.02~0.24、0.01~0.24、0.06~0.37、0.02~0.14、0.04~0.22、0.14~0.42、0.01~0.09、0~0.32 mg/g。经聚类分析、PCA和OPLS-DA发现,8批野生品(YS1~YS8)聚为一类,12批栽培品(ZP1~ZP12)聚为一类;半夏野生品的主要特征标志物为鸟苷、尿苷、腺苷、腺嘌呤,半夏栽培品的主要特征标志物为尿嘧啶、黄嘌呤、肌苷、β-胸苷。结论:建立了半夏药材中8种核苷类成分的含量测定方法;以核苷类成分为质量标志物可有效区分半夏野生品和栽培品,且野生品质量优于栽培品。

OBJECTIVE:To establish the method for the content determination of 8 nucleosides in wild and cultivated Pinellia ternate,and to conduct the difference analysis.METHODS:The contents of uracil,uridine,inosine,xanthine,adenine,guanosine,β-thymidine and adenosine in 20 batches of P.ternate(wild product YS1-YS8,cultivated product ZP1-ZP12)were determined by HPLC method.Based on the contents of the above 8 nucleosides,cluster analysis,principal component analysis(PCA),partial least squares analysis(OPLS-DA)were used to comprehensively evaluate the wild and cultivated P.ternata.RESULTS:The contents of uracil,uridine,inosine,xanthine,adenine,guanosine,β-thymidine and adenosine in 20 batches of P.ternate were 0.02-0.24,0.01-0.24,0.06-0.37,0.02-0.14,0.04-0.22,0.14-0.42,0.01-0.09,0-0.32 mg/g,respectively.Cluster analysis,PCA and OPLS-DA showed that 8 batches of wild P.ternate(YS1-YS8)were clustered into one category,and 12 batches of cultivated P.ternate(ZP1-ZP12)were clustered into one category.Main characteristic markers of wild P.ternate were guanosine,uridine,adenosine and adenine,while the main characteristic markers of cultivated P.ternate were urinine,xanthine,inosine,andβ-thymidine.CONCLUSIONS:The method for the content determination of 8 nucleosides in P.ternate is established.Nucleosides as quality markers can effectively distinguish wild and cultivated P.ternata,and the quality of the wild P.ternate was better than that of cultivated P.ternate.