基于石墨烯-金纳米粒子复合材料的黄芩苷电化学传感器的构建及应用

Construction and Application of Baicalin Electrochemical Sensor Based on Graphene-Au NPs Composites

通过原位还原法制备还原氧化石墨烯-金纳米粒子复合材料(Au NPs@rGO),并将其应用于构建具有高灵敏度的黄芩苷电化学传感器。由于rGO大的比表面积和Au NPs良好的导电能力,两者协同作用显著提高了黄芩苷的电化学响应。采用扫描电镜及电化学方法对修饰电极及黄芩苷的电化学行为进行了深入研究。在优化条件下,其差分脉冲伏安峰电流与黄芩苷浓度在0.05~10μmol/L范围内呈良好的线性关系,检出限(S/N=3)低至0.01μmol/L。将该方法应用于中药制剂中黄芩苷的检测,具有较好的精密度和准确度。

A new baicalin electrochemical sensor was developed based on reduced graphene oxide-gold nanocomposites(Au NPs@rGO),which was prepared by in-situ reduction method.Due to the large specific surface area of rGO and good conductivity of Au NPs,the synergistic effect of rGO combined with Au NPs significantly improved the electrochemical response of baicalin.The modified electrode was characterized by scanning electron microscopy and electrochemical method.Under the optimized conditions,there was a good linear relationship between peak current and concentration of baicalin over the range of 0.05-10μmol/L with a detection limit of 0.01μmol/L(S/N=3).The proposed sensor was applied for baicalin determination with excellent accuracy and satisfactory recovery in traditional Chinese medicine preparation,which provides a rapid and sensitive determination tool for quality control of baicalin in pharmaceutical preparations.