局域表面等离子体共振酶联免疫法的显色方法及检测应用

Colorimetric Methods and Detection Applications of Localized Surface Plasmon Resonance ELISA

随着现场实时快速检测技术的需求和发展,贵金属纳米颗粒由于具有独特的局域表面等离子体共振(LSPR)光学性能和优点(高稳定性、易于合成、易于表面修饰等),在疾病诊断、食品安全、环境质量检测领域有着广泛的应用空间。局域表面等离子体共振效应使得贵金属纳米颗粒在可见光范围内展示出丰富的色彩变化,并且这种色彩变化很容易通过改变贵金属颗粒的形状、大小、组成、周围介质环境进行调控。将这种可调控的显色与传统的酶联免疫吸附分析法(ELISA)相结合,构建局域表面等离子体共振ELISA,相比传统ELISA具有高灵敏度、快速、便携、可靠性高等优点。本文总结了局域表面等离子体共振ELISA显色的方法,并介绍其在疾病诊断、食品及环境检测等领域的比色检测进展,最后对局域表面等离子体共振ELISA当前存在的问题以及未来的发展方向进行了分析。

With the increasing requirements of point-of-care testing,noble metal nanoparticles have gained a wide range of applications in disease diagnosis,food safety and environmental detection due to their unique localized surface plasmon resonance properties and advantages(high stability,easy synthesis,easy surface modification,etc.).The localized surface plasmon resonance(LSPR)allows noble metal nanoparticles to exhibit rich color in the range of visible light,which is easily controlled by the shape,composition,size,and surrounding medium environment.By combining the tunable color change of noble metal nanoparticles and the traditional enzyme-linked immunoassay(ELISA),the localized surface plasmon resonance ELISA is created,which has the advantages of high sensitivity,portability and reliability compared with the traditional ELISA.This paper summarized the colorimetric methods of localized surface plasmon resonance ELISA and introduced the progress of their sensing applications in disease diagnosis,food safety and environmental detection.Finally,the current problems and future of localized surface plasmon resonance ELISA were discussed.