胆囊癌患者胆囊癌组织泛素结合酶E2T蛋白表达及其临床意义探讨

Implication of ubiquitinase E2T expression in cancerous tissues in patients with gallbladder cancer

目的探讨胆囊癌患者胆囊组织泛素结合酶E2T(UBE2T)蛋白表达及其临床意义。方法2015年1月~2017年12月收治的胆囊癌患者50例,手术后同时取癌组织和癌旁胆囊组织,另取良性疾病胆囊组织50例,采用Western blot法检测胆囊组织UBE2T蛋白相对表达水平,采用多因素Logistic回归模型分析影响生存的危险因素。结果胆囊癌组织UBE2T蛋白表达量为(2.9±0.4),显著高于癌旁组织(1.5±0.3,P<0.05)或正常胆囊组织(1.7±0.3,P<0.05);不同TNM肿瘤分期、浸润深度、淋巴结转移、远处转移和病理学分级癌组织UBE2T表达差异存在统计学意义(P<0.05);术后中位随访21.0个月,Logrank分析显示,31例UBE2T高表达和19例低表达胆囊癌患者中位生存时间分别为12.0个月和25.0个月(P<0.05);多元Logistic回归分析显示,TNM分期高[OR(95%CI)为1.9(1.5~2.4)]、UBE2T高表达[OR(95%CI)为2.5(2.1~2.9)]、肿瘤浸润程度高[OR(95%CI)为2.3(1.8~3.0)]、淋巴结转移程度高[OR(95%CI)为1.2(1.0~1.8)]、远处转移[OR(95%CI)为2.1(1.7~2.8)]和病理学分级高[OR(95%CI)为1.6(1.3~2.2)]是胆囊癌患者术后生存的危险因素(P<0.05)。结论胆囊癌患者胆囊组织UBE2T蛋白表达显著升高,可能与不良预后相关,有必要进行深入研究。

Objective This study aimed to explore the implication of ubiquitinase E2T(UBE2T)expression in cancerous tissues in patients with gallbladder cancer.Methods 50 patients with gallbladder cancer were included in this study between January 2015 and December 2017.The cancerous and adjacent noncancerous tissues as well as 50 normal gallbladder tissues were collected,and the relative expression of UBE2T was detected by Western bloting.All patients with gallbladder cancer underwent radical tumor resection and followed-up for up to 36 months.The multivariate Logistic regression analysis was applied to predict the risk factors for poor prognosis of patients with gallbladder cancer after operation.Results The relative expression ofUBE2T in cancerous tissue was(2.9±0.4),much higher than(1.5±0.3,P<0.05)in noncancerous or(1.7±0.3,P<0.05)in normal tissues;there were a significant differences as respect to the expression of UBE2T gallbladder between differentTNM stages,invasions,lymph node metastasis,remote metastasis and pathological classification(P<0.05);after 21.0 month follow-up,the Logrank analysis showed that there was a significant difference between 12.0 month survival in 31 patients with high cancerous UBE2T expression and 25.0 month(P<0.05)in 19 patients with low cancerous UBE2T expression;the multivariate Logistic analysis demonstrated that the poor TNM stage[OR(95%CI):1.9(1.5-2.4)],strong cancerous UBE2T expression[OR(95%CI):2.5(2.1-2.9)],tumor invasion[OR(95%CI):2.3(1.8-3.0)],lymph node metastasis[OR(95%CI):1.2(1.0-1.8)],remote metastasis[OR(95%CI):2.1(1.7-2.8)]and poor pathological classification[OR(95%CI):1.6(1.3-2.2)]were the risk factors for poor prognosis of patients with gallbladder cancer after operation(P<0.05).Conclusion The expression of UBE2T protein in gallbladder tissues of patients with gallbladder cancer is significantly intensified,which might be related to the poor prognosis.