结直肠癌中VASP表达及与转移关系研究

The expression of VASP in colorectal cancer and its relationship with metastasis

目的本研究旨在探讨VASP在结直肠癌中的表达,VASP与结直肠癌转移的关系。方法实时定量PCR及免疫组化方法分析VASP在结直肠癌组织中的表达量。通过体外Transwell实验及裸鼠体内尾静脉转移模型,分析VASP与结直肠癌转移的关系。结果 VASP在结直肠癌中的表达较癌旁明显升高(χ^(2)=82.1,P<0.001)。VASP与肿瘤大小、肿瘤分化、神经侵犯、淋巴结转移、远处转移、AJCC分期呈正相关。COX多因素分析表明VASP的表达是结直肠癌患者复发及死亡的独立预测因素之一。而体内外实验发现,过表达VASP可促进结直肠癌细胞的侵袭和转移。结论 VASP在结直肠癌中表达升高与结直肠癌预后差呈正相关,过表达VASP可促进结直肠癌的转移,是结直肠癌治疗的潜在靶点。

Objective We analyze the expression of VASP in CRC,and study the role of VASP in promoting CRC metastasis.Methods The expression of VASP was detected by Real-time PCR and immunohistochemistry in human CRC cohort.The effect of VASP-mediated invasion and metastasis were analyzed by Transwell assays and tail veins metastasis model both in vitro and in vivo.Results The expression levels of VASP were dramatically higher in CRC tissues than in adjacent nontumor tissues(χ^(2)=82.1,P<0.001).Elevated expression of VASP was positively correlated with tumor size,tumor differentiation,nerve invasion,the lymph node metastasis,distant metastasis,higher American Joint Committee on Cancer(AJCC stage)and poor prognosis in human CRC.COX analysis indicated VASP expression was independent predictor for high recurrence rate and poor overall survival.Elevated expression of VASP promotes CRC metastasis both in vitro and in vivo.Conclusion VASP was overexpression and correlated with poor prognosis in CRC.Upregulated VASP can promote CRC metastasis and could be served as a potential treatment marker for CRC patients.