波棱瓜子中1个新的香豆素

One new coumarin from seeds of Herpetospermum pedunculosum

研究波棱瓜子的化学成分。采用薄层色谱法、硅胶柱色谱法、Sephedax LH-20柱色谱法、半制备高效液相色谱法及重结晶等方法从波棱瓜子乙酸乙酯萃取部位中分离得到1个新的香豆素类化合物和2个已知的木脂素类化合物,根据其NMR,HR-ESI-MS等波谱数据鉴定为波棱内酯VⅢ(herpetolide H)(1)、phyllanglaucin B(2)、buddlenol E(3)。其中化合物1为新化合物,化合物2,3均为首次从波棱瓜属植物中分离得到。体外抗炎活性测试结果表明,herpetolide H对LPS诱导的RAW 264.7细胞有一定的NO抑制活性,其IC50为(46.57±3.28)μmol·L^(-1)。

This paper aims to investigate the chemical constituents of the seeds of Herpetospermum pedunculosum.One new coumarin and two known lignans were isolated from the ethanolic extract of the seeds of H.pedunculosum with thin layer chromatography(TLC),silica gel column chromatography,Sephedax LH-20 chromatography,Semi-preparative high performance liquid chromatography and recrystallization,etc.Their structures were elucidated as herpetolide H(1),phyllanglaucin B(2),and buddlenol E(3)by analysis of their physicochemical properties and spectral data.Among them,compound 1 was a new compound,and compounds 2 and 3 were isolated from this genus for the first time.In vitro anti-inflammatory activity test showed that herpetolide H had certain NO inhibitory activity for LPS-induced RAW 264.7 cells,with its IC50 value of(46.57±3.28)μmol·L^(-1).