miR-155、CD1d在胃腺癌患者外周血中的表达及其预后相关性研究

Expressions of miR-155 and CD1d in peripheral blood of patients with gastric adenocarcinoma and its correlation with prognosis

目的检测微小RNA-155(miR-155)和抗原递呈分子CD1d在胃腺癌患者外周血中表达情况,探讨二者的表达关系及临床意义。方法选择2016年1月~2017年6月就诊并进行胃腺癌手术治疗患者55例作为观察组,另选取同期进行健康体检人员56例作为对照组。采用qRTPCR法检测各研究对象外周血单个核细胞(PBMCs)miR-155表达水平,流式细胞仪检测表达CD1d的淋巴细胞百分数,分析二者与胃腺癌临床病理特征的关系,Pearson分析胃腺癌患者PBMCs中miR-155与CD1d表达水平的相关性,Kaplan-Meier分析胃腺癌患者术后3年生存情况,Cox分析影响胃腺癌患者预后的危险因素。结果miR-155在胃腺癌患者PBMCs中表达水平显著高于对照组;胃腺癌患者PBMCs中CD1d分子表达的细胞百分数显著低于对照组(P<0.05);miR-155、CD1d表达与肿瘤分化程度、TNM分期淋巴转移及浸润深度有关(P<0.05);胃腺癌患者PBMCs中miR-155和CD1d表达水平呈负相关(r=-0.585,P=0.000);miR-155高表达组患者的3年生存率显著低于miR-155低表达组,CD1d高表达组胃腺癌患者3年累积生存率显著高于低表达组患者(P<0.05);低分化、TNM分期、淋巴结转移、miR-155高表达、CD1d低表达是影响胃腺癌患者不良预后危险因素(P<0.05)。结论胃腺癌患者PBMCs中miR-155高表达、CD1d低表达,与患者生存质量有关,或可作为胃腺癌患者潜在的预后标志物。

Objective To detect the expressions of microRNA-155(miR-155)and antigen-presenting molecule CD1d in the blood of patients with gastric adenocarcinoma,and to explore their relationship and clinical significance.Methods A total of 55 patients with gastric adenocarcinoma treated in our hospital from January 2016 to June 2017 were selected as the as the observation group,and 56 healthy persons in the same period were selected as the control group.The expression level of miR-155 in peripheral blood mononuclear cells(PBMCs)was detected by qRT-PCR,the percentage of lymphocytes expressing CD1d was detected by flow cytometry,the relationship between them and clinicopathological features of gastric adenocarcinoma was analyzed,Pearson was uses to analyze the correlation between miR-155 and CD1d expression levels in PBMCs of patients with gastric adenocarcinoma,Kaplan-Meier was used to analyze the 3-year survival of patients with gastric adenocarcinoma,in addition,Cox analysis was used to analyze the risk factors affecting the prognosis of patients with gastric adenocarcinoma.Results The expression level of miR-155 in PBMCs of gastric adenocarcinoma patients was significantly higher than that of control group;the percentage of CD1d in PBMCs of gastric adenocarcinoma patients was significantly lower than that of control group(P<0.05);the expressions of miR-155 and CD1d were correlated with tumor differentiation,TNM stage,lymph node metastasis and depth of invasion(P<0.05);there was a negative correlation between miR-155 and CD1d in PBMCs of patients with gastric adenocarcinoma(r=-0.585,P=0.000);the 3-year survival rate of patients in high expression group of miR-155 was significantly lower than that in low expression group of miR-155,and the 3-year cumulative survival rate of gastric adenocarcinoma patients in high expression group of CD1d was significantly higher than that of low expression group(P<0.05);in addition,poor differentiation,TNM stage,lymph node metastasis,high expression of miR-155 and low expression of CD1d were risk factors of poor prognosis in patients with gastric adenocarcinoma(P<0.05).Conclusion The expression of miR-155 is high in PBMCs of patients with gastric adenocarcinoma,and the expression of CD1d is low,they are related to the quality of life of patients,and may be potential prognostic markers for gastric adenocarcinoma.