HPLC法同时测定蒙成药阿魏八味丸中5种化学成分的含量

Simultaneous determination of five components in mongolian medicine a wei-8 pill by HPLC

目的建立HPLC法同时测定蒙成药阿魏八味丸中五种活性成分(没食子酸、阿魏酸、丁香酚、木香烃内酯、去氢二异丁香酚)的含量。方法采用Thermo Hypersil GOLD C18色谱柱(250 mm×4.6 mm,5μm),流动相:甲醇(A)-0.05%磷酸水溶液(B),流速1.0 mL·min^(-1),进样量:10μL,柱温30℃,梯度洗脱程序:0~20 min:5%~60%A,20~45 min:60%A,45~60 min:70%A。检测波长:271 nm(没食子酸)、316 nm(阿魏酸)、280 nm(丁香酚)、225 nm(木香烃内酯)、280 nm(去氢二异丁香酚)。结果在建立的色谱条件下,没食子酸、阿魏酸、丁香酚、木香烃内酯、去氢二异丁香酚的质量浓度分别为8.00~80.00、2.05~20.45、360.0~3.60×103、40.40~404.0、8.01~80.10 mg·L-1,相关系数r均大于0.9990;方法的加样回收率为96.6%~102.7%,RSD≤2.07%。结论该方法可用于蒙成药阿魏八味丸的质量控制。

Objective To establish an HPLC method for the simultaneous determination of five components(gallic acid, ferulic acid, eugenol, costunolide, dehydroisoeugenol) in mongolian medicine A wei-8 pill.Method The mobile phase consisted of methanol(A)-0.05% phosphoric acid solution(B) with gradient elution on a Thermo Hypersil Gold C18 column(250 mm ×4.6 mm, 5μm) with a flow rate of 1.0 mL·min^(-1).The sample volume 10 μL and the column temperature 30 ℃.The gradient elution procedure: 0~20 min: 5%~60% A,20~45 min: 60% A,45~60 min: 70% A.Detection wavelength: 271 nm(gallic acid),316 nm(ferulic acid),280 nm(eugenol),225 nm(costunolide) 280 nm( dehydroisoeugenol).Results The linear ranges of gallic acid, ferulic acid, eugenol, costunolide and dehydroisoeugenol in mongolian medicine A wei-8 pill were 8.00~80.00、2.05~20.45、360.0~3.60×103、40.40~404.0、8.01~80.10 mg·L-1,respectively.The correlation coefficient r was greater than 0.9990.The average recoveries were 96.6%~102.7% and the RSD were less than or equal to 2.07%.Conclusion The method is simple, accurate and repeatable, and it can effectively control the quality of mongolian medicine a wei-8 pill.