摘要
在“碳中和”国家战略背景下,微藻减排烟气CO_(2)技术以其经济可持续的特点广受关注。微藻减排烟气CO_(2)高效、低成本和规模化发展过程中,为了提高螺旋藻过滤采收后循环液中NaHCO_(3)质量浓度,提高螺旋藻固定煤化工厂烟气CO_(2)速率,研制一种编制网式曝气器产生微米级CO_(2)气泡,延长烟气CO_(2)与循环液中Na2CO_(3)的反应时间,促进高效生成NaHCO_(3);通过双指示剂法测试藻液中Na2CO_(3)和NaHCO_(3)质量浓度,通过显微镜测试了螺旋藻生长过程中藻丝螺距和长度变化规律,通过植物效率分析仪测试螺旋藻细胞的叶绿素OJIP荧光诱导动力学曲线。研究结果表明:烟气CO_(2)通过编制网式曝气器反应后,跑道池中藻液的NaHCO_(3)质量浓度比相同条件下通过传统曝气条的NaHCO_(3)质量浓度提高43%;采用该循环液作为新鲜培养基,在跑道池中培养螺旋藻72 h后,螺旋藻的藻丝螺距和长度分别提高16%和12%,螺旋藻细胞暗适应下PSII最大量子产率和单位反应中心吸收光能分别提高21%和28%,最终导致螺旋藻生物质密度比未补充烟气CO_(2)前提高15%。烟气CO_(2)通过编制网式曝气器可转化为更多NaHCO_(3),从而促进了螺旋藻的生长固碳速率,为微藻固碳技术的高效、低成本规模化应用提供了技术设备选择。
In the context of national strategy of"carbon neutrality",the technology of CO_(2) fixation by microalgae receives much attention for its economic and sustainable characteristics.In order to increase the NaHCO_(3)mass concentration in the circulatory solution for improving the flue gas CO_(2) fixation rate from coal chemical plant by Spirulina biomass,a staggered woven mesh aerator was proposed.The circulatory solution was the residual solution after filtering and harvesting of Spirulina biomass solution.The staggered woven mesh aerator was used to produce the CO_(2) bubble with micrometer-grade bubble diameter,which can prolong the reaction time of CO_(2)bubble with Na2CO_(3) in the circulatory solution and produce higher NaHCO_(3) mass concentration.The mass concentrations of Na2CO_(3) and NaHCO_(3) in the microalgal solution were tested by the double indicator method,the changes of microalgal filament pitch and length during the growth of Spirulina were tested by microscopy,and the chlorophyll OJIP fluorescence-induced kinetic curves of Spirulina were tested by the Handy PEA.The results show that NaHCO_(3) mass concentration aerated to the staggered woven mesh aerator increases by 43% compared with that of the traditional rubber aerator.Taking this circulatory solution with higher NaHCO_(3) mass concentration generated at staggered woven mesh aerator as new culture medium and cultivating Spirulina biomass for 72 h,the trichome pitch and length of Spirulina increases by 16%and 12%,respectively.The maximum PSII quantum yield after dark adaption and the light absorption in the unit reaction center of Spirulina cells increases by 21%and 28%,respectively,which result in the increase of Spirulina biomass density by 15%compared with that before aerating coal chemical plant CO_(2) flue gas.Flue gas CO_(2) can be efficiently converted into NaHCO_(3) by compiling the staggered woven mesh aerator,thus promoting the CO_(2) fixation rate by Spirulina.It provides a technical equipment option for efficient and low-cost large-scale application of CO_(2) fixation by microalgae technology.
作者
郭王彪
刘书政
程军
乔占山
苏勇宁
郭彩凤
刘海军
李玉国
GUO Wangbiao;LIU Shuzheng;CHENG Jun;QIAO Zhanshan;SU Yongning;GUO Caifeng;LIU Haijun;LI Yuguo(State Key Laboratory of Clean Energy Utilization,Zhejiang University,Hangzhou 310027,China;Ordos Jiali Spirulina Co.Ltd,Ordos 016199,China;Inner Mongolia Rejuve Biotech Co.Ltd,Ordos 016199,China)
出处
《中南大学学报(自然科学版)》
EI
CAS
CSCD
北大核心
2021年第6期1944-1952,共9页
Journal of Central South University:Science and Technology
基金
国家重点研发项目(2016YFB0601003)
浙江省重点研发项目(2020C04006)。