罗汉果花绿原酸的提取和对DPPH·清除效果

Extraction of Chlorogenic Acid from Momordica grosvenorii Flower and Its Scavenging Effect on DPPH·

对罗汉果花中绿原酸提取工艺和体外抗氧化性进行研究,以便为罗汉果花的进一步开发利用提供理论依据。利用绿原酸的提取得率作为考察指标,通过单因素试验和正交试验确定罗汉果花绿原酸最佳提取工艺;通过对提取液进行DPPH·清除能力的测定来了解其体外抗氧化性。结果表明:以聚乙二醇为溶剂,采用微波辅助提取法效果较好,最佳提取工艺为聚乙二醇体积分数50%、料液比1∶35 g·mL^(-1)、微波功率350 W、提取时间5 min,此时绿原酸得率最高,为1.70%;当提取液中绿原酸的质量浓度为60μg/mL时,其对DPPH·清除率达到87%。罗汉果花中绿原酸可以作为一种重要的抗氧化剂用于食品中。

The extraction technology of chlorogenic acid from Momordica grosvenorii flower and its antioxidant activity in vitro were studied in order to provide theoretical basis for the further development and utilization of Momordica grosvenorii flower.Using the extraction yield of chlorogenic acid as the index,the best extraction process of chlorogenic acid from Momordica grosvenorii flower was determined by single factor test and orthogonal test.In order to know the antioxidant activity in vitro,DPPH·scavenging capacity of the extract was determined.The results showed that the microwave-assisted extraction method with polyethylene glycol as solvent had better effect.The best extraction process was polyethylene glycol concentration 50%,solid-liquid ratio 1∶35 g·mL^(-1),microwave power 350 W and extraction time 5 min.Under these conditions,the highest yield of chlorogenic acid was 1.70%.When the concentration of chlorogenic acid was 60μg/mL,the scavenging rate of DPPH·was 87%.Chlorogenic acid in Momordica grosvenorii flower could be used as an important antioxidant in food.