摘要
Since the outbreak of coronavirus disease 2019(COVID-19),it has become a global pandemic.The spike(S)protein of etiologic severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)specifically recognizes human angiotensin-converting enzyme 2(hACE2)as its receptor,which is recently identified as an interferon(IFN)-stimulated gene.Here,we find that hACE2 exists on the surface of exosomes released by different cell types,and the expression of exosomal hACE2 is increased by IFNα/β treatment.In particular,exosomal hACE2 can specifically block the cell entry of SARS-CoV-2,subsequently inhibit the replication of SARS-CoV-2 in vitro and ex vivo.Our findings have indicated that IFN is able to upregulate a viral receptor on the exosomes which competitively block the virus entry,exhibiting a potential antiviral strategy.
基金
supported by the National Special Research Program of China for Important Infectious Diseases(2018ZX10302103,2017ZX10202102-003,and 2018ZX10101004003001)
the Special 2019-nCov Program of Natural Science Foundation of China(NSFCK82041002)
the Important Key Program of Natural Science Foundation of China(81730060)
the National Natural Science Foundation of China(81701990)
the Joint innovation Program in Healthcare for Special Scientific Research Projects of Guangzhou(201803040002).
