急性痛风患者NALP3炎症小体表达及相关指标的价值研究

The value of NALP3 inflammasome expression and related indexes in patients with acute gout

目的探讨急性痛风患者NALP3炎症小体表达及相关指标的价值。方法选取2018年3月至2020年3月该院收治的152例急性痛风患者作为试验组,并选取同期体检健康者151例作为对照组。比较两组患者尿酸(UA)、空腹血糖(FPG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)、红细胞沉降率(ESR)、C反应蛋白(CRP),以及血清NALP3 mRNA和蛋白质的表达情况;采用多因素Logistic回归分析急性痛风的独立危险因素,采用Spearman相关分析NALP3炎症小体与UA、ESR、CRP的相关性。结果试验组UA、ESR、CRP水平高于对照组,差异有统计学意义(P<0.05);试验组NALP3蛋白、mRNA表达水平高于对照组,差异有统计学意义(P<0.05);多因素Logistic回归分析显示,NALP3炎症小体、UA、ESR和CRP是导致急性痛风的独立危险因素;Spearman相关分析显示,NALP3 mRNA的表达与患者UA、CRP、ESR呈正相关(r=0.559、0.548、0.727,P<0.001)。结论NALP3炎症小体在急性痛风患者中的表达水平明显升高,NALP3炎症小体及血清UA、ESR、CRP是急性痛风的独立危险因素,且NALP3炎症小体的表达水平与血清UA、ESR、CRP呈正相关。

Objective To investigate the expression of neutrophil NALP3 inflammasome in serum of patients with acute gout and the value of related indexes.Methods A total of 152 patients with acute gout admitted to the hospital from March 2018 to March 2020 were selected as the experimental group,and 151 healthy subjects during the same period were selected as the control group.The levels of uric acid(UA),fasting blood glucose(FPG),low density lipoprotein cholesterol(LDL-C),high density lipoprotein cholesterol(HDL-C),erythrocyte sedimentation rate(ESR),C-reactive protein(CRP),and serum NALP3 mRNA and protein were compared between the two groups.Multivariate Logistic regression was used to analyze the independent risk factors of acute gout,and Spearman correlation was used to analyze the correlation between NALP3 inflammasome and UA,ESR and CRP.Results UA,ESR and CRP levels in experimental group were higher than those in control group,and the difference was statistically significant(P<0.05).The protein and mRNA expression levels of NALP3 in experimental group were higher than those in control group,and the differences were statistically significant(P<0.05).Multivariate Logistic regression analysis showed that NALP3 inflammasome,UA,ESR and CRP were independent risk factors for acute gout.Spearman correlation analysis showed that NALP3 mRNA expression was positively correlated with UA,CRP and ESR(r=0.559,0.548,0.727,P<0.001).Conclusion The expression level of NALP3 inflammasome was significantly increased in patients with acute gout.NALP3 inflammasome and serum UA,ESR and CRP were independent risk factors for acute gout,and the expression level of NALP3 inflammasome was positively correlated with serum UA,ESR and CRP.