摘要
目的:探讨人脐带间充质干细胞(Human umbilical cord mesenchymal stem cells,hUC-MSCs)对脊柱骨折大鼠愈合及神经功能的影响。方法:脊柱骨折Sprague-Dawley雄性大鼠模型30只随机分为hUC-MSCs组与对照组,各15只。hUC-MSCs组大鼠在骨折部位移植0.5 mL的hUC-MSCs(细胞浓度为2×10^(6)/mL),对照组大鼠移植同体积的生理盐水,记录大鼠愈合及神经功能变化情况。结果:两组造模后15 min、30 min、90 min的平均动脉压都波动明显,不过组间对比差异无统计学意义(P>0.05)。与造模后2 w对比,两组造模后4 w的神经功能BBB评分均升高,且hUC-MSCs组造模后2 w、4 w的神经功能BBB评分都高于对照组(P<0.05)。hUC-MSCs组造模后8 w的骨体积分数高于对照组(P<0.05)。hUC-MSCs组骨折部位附近有少量骨痂生长,骨折线逐渐消失;骨痂已明显包裹骨折部位。hUC-MSCs组造模后8 w的脊髓细胞凋亡指数低于对照组(P<0.05)。结论:hUC-MSCs在脊柱骨折大鼠的应用能促进骨折愈合与改善神经功能,也可以抑制脊髓细胞凋亡,从而发挥很好的治疗作用。
Objective:To investigate the effects of human umbilical cord mesenchymal stem cells(hUC-MSCs)on spinal fracture healing and nerve function in rats.Methods:30 cases of Sprague-Dawley male rat models of spinal fracture were randomly divided into h UC-MSCs group and control group with 15 rats in each groups.Rats in the hUC-MSCs group were transplanted with 0.5 mL of h UC-MSCs(cell concentration of 2×10^(6)/mL)at the fracture site,and the rats in the control group were transplanted with the same volume of saline.The healing and nerve function changes of the rats were recorded.Results:The mean arterial pressures of the two groups were fluctuated significantly at 15 min,30 min,and 90 min after modeling,but the difference compared between the groups were not statistically significant(P>0.05).Compared with 2 weeks after modeling,the neurological function BBB scores of the two groups increased 4 weeks after modeling,and the neurological function BBB scores of the hUC-MSCs group were higher than those of the control group 2 and 4 weeks after modeling(P<0.05).The bone volume fraction of hUC-MSCs group 8 weeks after modeling were higher than that of control group(P<0.05).In the hUC-MSCs group,there were small amount of callus growing near the fracture site,and the fracture line gradually disappeared;the callus were obviously wrapped the fracture site.The apoptosis index of spinal cord cells in the hUC-MSCs group were lower than that in the control group at 8 weeks after modeling(P<0.05).Conclusion:The application of hUC-MSCs in rats with spinal fractures can promote fracture healing and improve nerve function,as well as inhibit spinal cord cell apoptosis,thereby exert good therapeutic effect.
作者
卫永鲲
雷涛
赵利明
马慧玲
杨斌辉
WEI Yong-kun;LEI Tao;ZHAO Li-ming;MA Hui-ling;YANG Bin-hui(Department of Orthopedics,3201 Hospital Affiliated to Xi'an Jiaotong University School of Medicine,Hanzhong,Shaanxi,723000,China;Department of Military Biomedical Engineering,Air Force Military Medical University,Xi'an,Shaanxi,720000,China;Department of Orthopedics,Baoji High-tech Hospital,Baoji,Shaanxi,721000,China;Department of Medicine,Hanzhong Vocational and Technical College,Hanzhong,Shaanxi,723000,China)
出处
《现代生物医学进展》
CAS
2021年第12期2212-2215,共4页
Progress in Modern Biomedicine
基金
国家自然科学基金青年基金项目(51907197)
陕西省科技厅基金项目(2019SF-168)。
关键词
人脐带间充质干细胞
脊柱骨折
骨折愈合
神经功能
细胞凋亡
Human umbilical cord mesenchymal stem cells
Spinal fractures
Fracture healing
Nerve function
Apoptosis