人脐带间充质干细胞对脊柱骨折大鼠愈合及神经功能的影响

Effects of Human Umbilical Cord Mesenchymal Stem Cells on the Healing and Nerve Function of Spinal Fracture Rats

目的:探讨人脐带间充质干细胞(Human umbilical cord mesenchymal stem cells,hUC-MSCs)对脊柱骨折大鼠愈合及神经功能的影响。方法:脊柱骨折Sprague-Dawley雄性大鼠模型30只随机分为hUC-MSCs组与对照组,各15只。hUC-MSCs组大鼠在骨折部位移植0.5 mL的hUC-MSCs(细胞浓度为2×10^(6)/mL),对照组大鼠移植同体积的生理盐水,记录大鼠愈合及神经功能变化情况。结果:两组造模后15 min、30 min、90 min的平均动脉压都波动明显,不过组间对比差异无统计学意义(P>0.05)。与造模后2 w对比,两组造模后4 w的神经功能BBB评分均升高,且hUC-MSCs组造模后2 w、4 w的神经功能BBB评分都高于对照组(P<0.05)。hUC-MSCs组造模后8 w的骨体积分数高于对照组(P<0.05)。hUC-MSCs组骨折部位附近有少量骨痂生长,骨折线逐渐消失;骨痂已明显包裹骨折部位。hUC-MSCs组造模后8 w的脊髓细胞凋亡指数低于对照组(P<0.05)。结论:hUC-MSCs在脊柱骨折大鼠的应用能促进骨折愈合与改善神经功能,也可以抑制脊髓细胞凋亡,从而发挥很好的治疗作用。

Objective:To investigate the effects of human umbilical cord mesenchymal stem cells(hUC-MSCs)on spinal fracture healing and nerve function in rats.Methods:30 cases of Sprague-Dawley male rat models of spinal fracture were randomly divided into h UC-MSCs group and control group with 15 rats in each groups.Rats in the hUC-MSCs group were transplanted with 0.5 mL of h UC-MSCs(cell concentration of 2×10^(6)/mL)at the fracture site,and the rats in the control group were transplanted with the same volume of saline.The healing and nerve function changes of the rats were recorded.Results:The mean arterial pressures of the two groups were fluctuated significantly at 15 min,30 min,and 90 min after modeling,but the difference compared between the groups were not statistically significant(P>0.05).Compared with 2 weeks after modeling,the neurological function BBB scores of the two groups increased 4 weeks after modeling,and the neurological function BBB scores of the hUC-MSCs group were higher than those of the control group 2 and 4 weeks after modeling(P<0.05).The bone volume fraction of hUC-MSCs group 8 weeks after modeling were higher than that of control group(P<0.05).In the hUC-MSCs group,there were small amount of callus growing near the fracture site,and the fracture line gradually disappeared;the callus were obviously wrapped the fracture site.The apoptosis index of spinal cord cells in the hUC-MSCs group were lower than that in the control group at 8 weeks after modeling(P<0.05).Conclusion:The application of hUC-MSCs in rats with spinal fractures can promote fracture healing and improve nerve function,as well as inhibit spinal cord cell apoptosis,thereby exert good therapeutic effect.