circTCF25抑制mTOR信号通路对TNF⁃α诱导血管平滑肌细胞增殖和迁移的影响

Effect of circTCF25 on the proliferation and migration of TNF⁃αinduced vascular smooth muscle cells by inhibiting mTOR signaling pathway

目的探讨环状RNA TCF25(circTCF25)对肿瘤坏死因子α(TNF⁃α)诱导血管平滑肌细胞(A7r5)增殖和迁移的影响和可能机制。方法TNF⁃α(100 ng/mL)诱导A7r5后,RT⁃qPCR检测circTCF25、mTOR mRNA和p70S6k mRNA表达,CCK⁃8法和集落形成实验检测细胞增殖,Transwell实验检测细胞迁移。将circTCF25过表达载体转染A7r5,检测过表达circTCF25对TNF⁃α处理的A7r5增殖、迁移、mTOR mRNA和p70S6k mRNA表达的影响。结果TNF⁃α诱导后A7r5增殖能力、迁移细胞数显著升高,circTCF25表达显著降低,mTOR mRNA和p70S6k mRNA表达显著升高(P<0.05);过表达circTCF25后,TNF⁃α诱导的A7r5增殖能力、迁移细胞数显著降低,mTOR mRNA和p70S6k mRNA表达显著降低(P<0.05)。抑制mTOR信号通路可减轻过表达circTCF25对TNF⁃α作用的A7r5增殖和迁移的影响(P<0.05)。结论circTCF25能抑制TNF⁃α诱导的血管平滑肌细胞增殖和迁移,其机制可能与阻断mTOR信号通路有关。

Objective To investigate the effect of circular RNA TCF25(circTCF25)on the proliferation and migration of vascular smooth muscle cells(A7r5)induced by tumor necrosis factorα(TNF⁃α)and explore its possible mechanism.Methods After TNF⁃α(100 ng/mL)induced A7r5,RT⁃qPCR was used to detect the expression of circTCF25,mTOR mRNA and p70S6k mRNA.CCK⁃8 assay and colony formation assay were used to detect cell proliferation,and Transwell assay was used to detect cell migration.A7r5 were transfected with circTCF25 overexpression vector,and the effects of circTCF25 overexpression on the proliferation,migration,mTOR mRNA and p70S6k mRNA expression of TNF⁃α⁃treated A7r5 were detected by the above methods.Results After TNF⁃αinduction,the proliferation ability of A7r5 and the number of migrated cells were significantly increased,the expression of circTCF25 was significantly reduced,and the expression of mTOR mRNA and p70S6k mRNA was significantly increased(P<0.05).After overexpression of circTCF25,the proliferation ability was significantly impaired and the number of migrating cells was significantly reduced,and the expressions of mTOR mRNA and p70S6k mRNA of A7r5 induced by TNF⁃αwere significantly reduced(P<0.05).Inhibition of the mTOR signaling pathway could alleviate the effect of circTCF25 overexpression on the activity and migration of A7r5 induced by TNF⁃α(P<0.05).Conclusions circTCF25 can inhibit the proliferation and migration of vascu⁃lar smooth muscle cells induced by TNF⁃α,for it may inhibit mTOR signaling pathway in mechanism.