大麦虫取食塑料影响因素及肠道功能菌群探究

Research on Influencing Factors of Polystyrene Consumption by Zophobas atratus Larvae,and Gut Core Functional Microbiome

大麦虫取食降解泡沫塑料易受环境因子的影响而导致存活率降低.为了减少环境因子对塑料降解研究的干扰,通过条件实验探究了环境因素对大麦虫幼虫生长和取食泡沫塑料的影响,借助MiSeq测序解析了其消化道内潜在的塑料降解核心功能菌群.实验结果表明,光照、塑料密度与接触面积均对大麦虫生长和取食聚苯乙烯(Polystyrene,PS)泡沫塑料有明显的影响.黑暗条件有利于大麦虫的生长和取食塑料;密度低且接触面积小的PS泡沫塑料更容易被大麦虫取食,但是破碎程度高的泡沫塑料不利于大麦虫的取食,而仅以PS泡沫塑料作为大麦虫的唯一食物无法满足其幼虫正常生长的需求.取食PS泡沫塑料对其消化道的菌群结构影响显著,unclassified Enterobacteriaceae,Dysgonomonas,Sphingobacterium和Enterococcus 4个菌属与PS泡沫塑料降解密切相关.

The survival rate of Zophobas atratus in the styrofoam biodegradation investigation is affected by environmental factors.In order to reduce the interference on the experiments of plastic biodegradation,effects of environmental factors on the growth of Zophobas atratus and consumption of the styrofoam were investigated,and the potential core functional bacteria was speculated by using the MiSeq sequencing technology.The experimental results indicated that light condition,plastic density and contact area have significant impacts on the growth of Zophobas atratus and the consumption of polystyrene(PS)styrofoam.Dark condition is conducive to the growth and plastic consumption by Zophobas atratus.Styrofoam with low density and low contact area is favored by Zophobas atratus.However,plastic with high breakage degree is not conducive to Zophobas atratus,and taking styrofoam as sole diet can not meet the growth requirements of larvae.Significant changes in gut microbiome of Zophobas atratus was observed with PS styrofoam diet,and genera of unclassified Enterobacteriaceae,Dysgonomonas,Sphingobacterium and Enterococcus are closely related to the degradation of PS styrofoam.