摘要
以玉米农田土壤为筛选样本,以玉米秸秆为筛选碳源,通过多重筛选试验组合,成功构建一组高效降解纤维素的复合型菌剂Y8,并进行室内摇瓶试验、室外对比性坑试,从实际应用的角度探讨菌剂Y8的降解能力。经菌落和菌体形态观察及生理生化初步鉴定,这8株纤维素降解菌株均属于芽孢杆菌属(Bacillus sp.)。在摇瓶试验中,该菌剂对玉米秸秆的降解率达44.70%;田间试验中菌剂对秸秆的降解率可达60%以上。研究结果表明,菌剂Y8可明显提高玉米秸秆田间原位腐解效率,并在一定程度上抑制植物病原菌的生长。
Taking corn farmland soil as the screening sample and corn straw as the carbon source,through multiple screening experiments,a group of compound bacterifier Y8 with high efficiency for cellulose degradation was successfully constructed.The indoor shaking bottle experiment and outdoor comparative pits were conducted to explore the degradation capacity of bacterifier Y8 from the perspective of practical application.The morphological observation and physiological and biochemical identification of the colonies and bacteria showed that all the 8 cellulose degrading strains belonged to Bacillus sp..In the flask shaking experiment,the degradation rate of the bacterial agent to corn straw was 44.70%.In the field experiment,the degradation rate of bacterial agent to straw could reach more than 60%.The research results showed that the fungus Y8 could significantly improve the efficiency of corn stalk decomposing in situ in the field,and inhibit the growth of plant pathogenic bacteria to a certain extent.
作者
徐杰
王泽懿
刘文越
狄鲁滨
黄雨阳
XU Jie;WANG Ze-yi;LIU Wen-yue(College of Resources and Environment,Northeast Agricultural University,Harbin,Heilongjiang 150030)
出处
《安徽农业科学》
CAS
2021年第13期74-79,共6页
Journal of Anhui Agricultural Sciences
关键词
复合型菌剂
原位腐解
酶解活力
玉米秸秆
研制
功能验证
Compound bacteria agent
Decomposing in situ
Enzyme activity
Corn straw
Development
Functional verification