C14ORF169在结直肠癌中的表达及对化疗和预后的影响

Expression of C14ORF169 in colorectal cancer and its effect on chemotherapy and prognosis

目的:探索C14ORF169在结直肠癌组织中的表达和对化疗及预后的影响。方法:利用Oncomine数据库分析C14ORF169在结直肠腺瘤、结直肠癌与相应正常组织中的表达水平差异。采用GEO数据库中结直肠癌样本数据集GSE12945和GSE17536分析C14ORF169表达水平与结直肠癌患者临床病理特点及预后的关系。构建C14ORF169高表达和敲减的结直肠癌细胞系,采用CCK-8法检测5-氟尿嘧啶(5-FU)半抑制浓度(IC50)。结果:C14ORF169在结直肠腺瘤和结直肠癌组织中的表达水平均高于相应的正常组织,C14ORF169高表达结直肠癌患者总生存时间和疾病特异性生存时间明显短于低表达患者(P<0.05),C14ORF169高表达与结直肠癌AJCC分期密切相关(P<0.05)。敲减C14ORF169后的结直肠癌细胞对5-FU敏感性增加,反之则化疗耐药。结论:C14ORF169在结直肠癌中呈高表达,并与患者化疗耐药及预后不良相关。

Objective:To explore the expression of C14ORF169 in patients with colorectal cancer,and analyze its impact on efficacy of chemotherapy and prognosis.Methods:C14ORF169 differences in expression level of colorectal adenoma,colorectal cancer and corresponding normal tissues were compared using Oncomine database.The correlation of C14ORF169 expression with patients′clinicopathological characteristics and prognosis was analyzed by using the GSE12945 and GSE17536 colorectal cancer datasets downloaded from the gene expression omnibus(GEO)database.The C14ORF169 over expression and knockdown colorectal cancer cell lines were constructed respectively,and the 5-fluorouracil(5-FU)half-maximal inhibitory concentration(IC50)was detected by using CCK-8 method.Results:The level of C14ORF169 in colorectal adenomas and colorectal cancer tissues were significantly higher than that in normal tissues(P<0.05).Overall survival and colorectal cancer specific survival of patients with high expression of C14ORF169 were significantly shorter than those with low C14ORF169 expression(P<0.05).High expression of C14ORF169 was closely related to AJCC advanced stage of colorectal cancer.The sensitivity of colorectal cancer cells to 5-FU increased after C14ORF169 knock-down,while C14ORF169 over-expression cell lines showed enhanced chemoresistance.Conclusion:C14ORF169 is highly expressed in colorectal cancer,which is associated with chemotherapy resistance and poor prognosis.