摘要
以聚多巴胺(PDA)为黏结剂,在硅、玻璃和金的表面制备了由具有抗蛋白质吸附功能的聚(2-甲基-2-噁唑啉)(PMOXA)和具有刺激响应性的聚丙烯酸(PAA)组成的混合聚合物刷。通过X-射线光电子能谱(XPS)、可变角光谱椭偏仪(VASE)对其进行了表征,并利用水接触角(WCA)研究了聚合物刷表面的亲/疏水性。选取pH=9、I=0.01 mol/L(I为离子强度)作为纤维蛋白吸附条件,pH=9、I=0.15 mol/L作为脱附条件,用荧光显微镜和表面等离子共振(SPR)分别定性和定量地研究了混合聚合物刷对纤维蛋白的吸附-脱附行为。结果表明,当pH=9,离子强度由0.01 mol/L向0.15 mol/L转变时,混合聚合物刷表面会从相对疏水状态转变为亲水状态;增加PMOXA的聚合度会减少混合聚合物刷对蛋白质的吸附量,同时也会明显改善混合聚合物刷对蛋白质的脱附率;聚合度60的PMOXA和聚合度90的PAA以质量比3∶2顺序接枝制备的混合聚合物刷实现了对纤维蛋白83.5%的脱附率。
The mixed polymer brushes which combine poly(2-methyl-2-oxazoline)(PMOXA)with anti-protein adsorption properties and poly(acrylic acid)(PAA)with stimulus-responsive properties were prepared on the material surfaces(including glass,silicon,and gold)by using poly(dopamine)(PDA)as an anchor.The surface chemical composition and thickness of the mixed polymer brushes were characterized by X-ray photoelectron spectroscopy(XPS)and variable angle spectroscopic ellipsometry(VASE).The hydrophilicity of the polymer brush surface was studied by water contact angle(WCA).Choosing pH=9 and I=0.01 mol/L(I is ionic strength)as the fibrinogen adsorption conditions,and pH=9 and I=0.15 mol/L as the fibrinogen desorption conditions.The adsorption and desorption behavior of fibrinogen on mixed polymer brushes was studied qualitatively and quantitatively by fluorescence microscope and surface plasmon resonance(SPR).The results showed that when the environmental pH=9 and I changes from 0.01 mol/L to 0.15 mol/L,the surface of the mixed polymer brushes would change from a relatively hydrophobic state to a hydrophilic state.Increasing the degree of polymerization of PMOXA would reduce the amount of protein adsorption of the mixed polymer brushes,and would also significantly improve the protein desorption rate of the mixed polymer brushes.The mixed polymer brush prepared by sequential grafting PMOXA with a degree of polymerization of 60 and PAA with a degree of polymerization of 90 in a mass ratio of 3∶2 achieved the desorption rate of 83.5%for fibrinogen.
作者
胡飞
朱良宇
王雨晨
何康
ATIF Muhammad
王延梅
HU Fei;ZHU Liangyu;WANG Yuchen;HE Kang;ATIF Muhammad;WANG Yanmei(School of Chemistry and Materials Science,University of Science and Technology of China,Hefei 230026,China)
出处
《功能高分子学报》
CAS
CSCD
北大核心
2021年第4期352-361,共10页
Journal of Functional Polymers
基金
国家自然科学基金(21674102)。