基于超支化聚硫醚构建非水相生物黏合剂

Non-Aqueous Bioadhesive Based on Hyperbranched Polythioether

以2-(丙烯酰氧基)乙基甲基丙烯酸酯为AA'单体,三羟甲基丙烷三(3-巯基丙酸酯)为B3单体,通过硫醇-(甲基)丙烯酸酯迈克尔加成聚合反应,制备了富含硫醇基团、室温为液态的超支化聚硫醚(HBP)。将HBP与聚乙二醇二丙烯酸酯(PEGDA)混合制备了黏合剂。利用小瓶倾斜法、流变测试、拉伸搭接剪切测试、溶胀-降解测试和体外细胞毒性测试等研究了不同PEGDA添加量对黏合剂性能的影响。结果表明:这种非水相生物黏合剂能在水环境中使用,对猪皮的黏合强度最高可达43 kPa,且具有优异的细胞相容性。

As a substitute of suture,bioadhesive attracts much attention in clinical application.However,the development of bioadhesive with excellent bonding performance for underwater usage remains a challenge.Herein,a non-aqueous bioadhesive with underwater bonding properties was manufactured based on hydrophobic hyperbranched polythioether(HBP).HBP with the surface containing abundant thiol groups was synthesized through a simple thiol-(meth)acrylate Michael addition polymerization of 2-(acryloyloxy)ethyl methacrylate(AA'monomer)and trimethylolpropane tris(3-mercaptopropionate)(B3 monomer).HBP was a liquid material under room temperature,thus enabling the manufacturing of non-aqueous adhesives(HBP-x)by the direct addition of poly(ethylene glycol)diacrylate.The properties of HBP-x were studied using vial-tilt method,rheological test,lap-shear adhesion test,swelling test,degradation test and in vitro cytotoxicity test.Results showed that the HBP-adhesive could bond porcine skin with an underwater adhesive strength of up to 43 kPa.Moreover,the HBP-adhesive minimally swelled(7.2%—17.3%)and was degradable.In vitro cell experiments showed that the HBP-adhesive showed very low cytotoxicity to L929 cells,and allowed the proliferation of L929 cells on the surface of the adhesive.This non-aqueous HBP-adhesive based on thiol-acrylate Michael addition reaction displayed an underwater bonding ability and excellent cytocompatibility,holding great potential for clinical applications.