野生黄精离体快繁技术体系的优化

Optimization of Rapid Propagation Technology of Polygonatum sibiricum

以野生黄精不定芽为材料,以MS为基本培养基,添加不同浓度的植物生长调节剂6-BA、2,4-D、NAA、KT进行3因素4水平的正交设计试验,筛选黄精增殖的最佳培养条件;探讨不同培养温度对黄精组培苗继代增殖的影响;以1/2 MS为基本培养基,探讨不同生长素及其浓度和不同培养容器对组培苗生根的影响。结果表明,黄精组培苗最佳继代增殖培养基为MS+2.0 mg/L 6-BA+0.2 mg/L 2,4-D+0.4 mg/L NAA,最佳培养温度为22℃,平均繁殖系数达6.88;最佳生根培养基为1/2 MS+0.5 mg/L NAA+0.2 mg/L IBA,平均生根率达96.2%;接种袋是黄精工厂化育苗的首选生根培养容器。

In order to establish the optimum culture condition of Polygonatum sibiricum,the adventitious buds were used as the explants and MS as the basic medium to study the effects of different culture temperature and plant growth regulators(6-BA,2,4-D,NAA,KT)with an orthogonal experimental design L9(34)on multiplication and plant regeneration in vitro.1/2 MS was used as the basic medium to study the effects of different plant growth regulators and culture vessel on rooting of tissue cultured plantlets.The optimal multiplication medium was MS+2.0 mg/L 6-BA+0.2 mg/L 2,4-D+0.4 mg/L NAA.The optimal culture temperature was 22℃and the average proliferation coefficient reached 6.88.The optimal rooting medium was 1/2 MS+0.5 mg/L NAA+0.2 mg/L IBA,and the average frequency of shoot rooting reached 96.2%.Inoculation bag was the first choice culture vessel for large-scale production of P.sibiricum.