5个抗病基因功能标记在234份番茄材料中的检测

Detection of Five Disease Resistance Genes in 234 Tomato Materials with Functional Markers

番茄是世界上最重要的蔬菜作物之一,病害的发生和蔓延使番茄生产受到严重影响,培育多抗番茄品种是防治病害最为经济和有效的方法。本研究利用功能标记对234份番茄材料进行Cf-9、Mi-1、I-2、Ph-3和Tm-2~a等5个抗病基因的检测,发现其中含Cf-9的番茄材料为136份,含Mi-1的材料为20份,含I-2的材料为124份,含Ph-3的材料38份,含Tm-2~a的材料为24份。234份番茄材料中,不含这5个抗病基因的材料有29份;含1个抗病基因的材料有106份;含2个抗病基因的材料有70份;含3个抗病基因的材料有23份;含4个抗病基因的材料有6份,分别为11CT387-2M、11CHT164-2、11CT150、11CT271、11CT730和11CT774;同时含这5个抗性基因的番茄材料尚未检测到。该研究结果将为进一步开展多个抗病基因的聚合育种提供参考依据。

Tomato is one of the most important vegetable crops in the world.Tomato production is seriously affected by the occurrence and spread of diseases.It is the most economical and effective way to control diseases by cultivating varieties with multiple resistant genes to different diseases.Functional markers were used to test the appearance of Cf-9,Mi-1,I-2,Ph-3 and Tm-2a genes in 234 tomato materials.Cf-9 was found in 136 materials.Mi-1 was found in 20 materials.I-2 was found in 124 materials.Ph-3 was found in 38 materials.And Tm-2a was found in 24 materials.Among the 234 tomato materials,29 did not contain any of these five resistant genes,106 contained one resistant gene,70 contained two resistant genes,23 contained three resistant genes,6 contained four resistant genes(line 11CT387-2M,11CHT164-2,11CT150,11CT271,11CT730 and 11CT774).None of the materials had five resistant genes.The results would lay a foundation for resistance genes pyramiding in the future.