破壁法用于马勃生物碱的提取研究

Study on Extraction of Alkaloids from Puffball by Wall-breaking Method

采用“酸性醇回流法”提取马勃生物碱,并首次引入球磨机破壁技术提高马勃生物碱粗品的提取率,通过改变破壁转速和回流提取时间以确定最优提取方案。实验发现破壁工序有利于马勃生物碱的提取,在一定范围内,提取率随破壁转速的提高而增大;回流提取时间影响生物碱的提取率,提取率随提取时间呈先递增后递减变化;在实验范围内,破壁转速为500 rpm,提取时间为3 h,马勃生物碱粗品的提取率最高。

Acid alcohol reflux method was applied to extract alkaloids from puffball,and the wall-breaking technology was introduced for the first time to improve the extraction efficiency.The optimal extraction method of alkaloids from puffball was determined by changing wall breaking speed and the reflux extraction time.It was found that the wall breaking process could improve the extraction efficiency of alkaloids,and the extraction efficiency increased with the improved wall breaking speed in a certain range.Reflux extraction time also affected the extraction efficiency of alkaloids,the extraction efficiency increased and then decreased with the improved extraction time.The highest extraction amounts of crude alkaloids from puffball was conducted with the wall breaking speed of 500 rpm and the extraction time of 3 h.