IAA高产菌-农杆菌(Agrobacterium)对小球藻生长代谢的影响

Effects of IAA-producing bacterium-Agrobacterium on the growth and metabolism of Chlorella

以一株分离自小球藻培养体系中的IAA高产菌-农杆菌(Agrobacterium)为研究对象,从生物量积累-抗氧化系统响应-转录组学不同层次揭示了农杆菌对藻细胞生长代谢的影响机制.结果表明,农杆菌与相当浓度水平的外源IAA相比,对小球藻的生长具有更高的促进作用;农杆菌可提高小球藻可溶性糖和蛋白的含量,分别达到纯藻体系的1.34倍和1.43倍;农杆菌可显著提高小球藻SOD活性和降低MDA的含量.转录组学分析结果显示:对基因进行GO功能分类注释,细胞组分包含的unigene最多.与纯藻体系相比,添加细菌体系和添加外源IAA体系分别有246和966个基因显著下调,有1394和1115个基因显著上调,且共同的差异基因有948个.

In this study,an IAA-producing bacterium strain-Agrobacterium isolated from the Chlorella culture system was used as the research object,and the influence mechanism of IAA-secreting bacteria on the microalgal growth and metabolism of microalgal cells from certain levels of biomass accumulation,antioxidant system response and transcriptomics was revealed.The results showed that compared with the addition of the same level of concentration of exogenous IAA,the addition of Agrobacterium had a greater promoting effect on the growth of Chlorella.In addition,Agrobacterium enhanced the contents of soluble sugar and protein in Chlorella by 1.34 times and 1.43 times of that of pure algal system.Similarly,Agrobacterium significantly increased SOD activity and decreased MDA content in Chlorella.Transcriptomics analysis showed that GO function classification annotation was carried out for genes,in which cellular component contained prominently the unigene.Compared with Chlorella,246 and 966 genes were significantly down-regulated in bacterial and exogenous IAA system,while 1394 and 1115 unigene were expressively up-regulated.Also,there were 948 common differential genes.