PRF联合MTA用于兔牙直接盖髓术的影像学研究

Radiographic Study of PRF Combined with MTA for Direct Pulp Capping of Rabbit Teeth

目的:探讨PRF膜联合MTA用于兔下颌门齿直接盖髓术后修复性牙本质形成情况。方法:将32只健康新西兰兔随机分为4组,每组8只,每只实验兔的2颗下颌门齿作为实验牙且接受同一种处理方法,根据盖髓材料不同分为:3个实验组(PRF+MTA组、PRF组、MTA组)、空白对照组;建立实验牙露髓模型,根据分组不同应用不同盖髓材料进行直接盖髓术;分别在术后第7天、第28天从每组中随机选择4只实验兔并处死,拍摄CBCT,运用Mimics19.0软件对实验牙进行三维重建,测量术后第7天、第28天穿髓孔处形成的钙化物的体积。结果:术后第7天,3个实验组形成钙化物体积均比空白对照组大,差异有统计学意义(P<0.05),但3个实验组组间两两比较差异无统计学意义(P>0.05);术后第28天,PRF+MTA组形成钙化物体积较PRF组、空白对照组大,差异有统计学意义(P<0.05),PRF+MTA组形成钙化物体积比MTA组大,但两组间差异无统计学意义(P>0.05)。结论:从影像学角度观察PRF联合MTA促进修复性牙本质形成的水平与MTA无差异,都处于较高水平,但生成的修复性牙本质的性质如何、牙髓状态如何需要组织学观察进行深入研究。

Objective:To investigate the ability for pulp to form hard tissue when PRF membrane combined with MTA was used as a new compound pulp capping agent for direct pulp capping.Methods:32 New Zealand rabbits were randomly divided into 4 groups with 8 experimental rabbits in each group and central mandibular incisors of each rabbit were used as experimental teeth and received the same treatment.According to the different capping materials,they were divided into three experimental groups(PRF+MTA group,MTA group,PRF group)and blank control group;establishment the dental pulp exposure model and direct pulp capping.The experimental teeth were filmed with CBCT immediately before and after surgery,on the 7th and 28th day,and Mimics19.0 software was used to reconstruct the experimental teeth and calculate the volume of dentine bridge formed at the perforating pulp hole.Results:On 7th day after surgery,the dentine bridge volumes in three experimental groups were all larger than those in the blank control group(P<0.05),but there was no significant difference among three experimental groups(P>0.05).On 28th day after surgery,the dentine bridge volume of PRF+MTA group was larger than that of PRF group and blank control group(P<0.05),but there was no statistically significant difference between the PRF+MTA group and MTA group(P>0.05).Conclusion:From the perspective of imaging,the level of PRF combined with MTA in promoting the formation of restorative dentin was not different from that of MTA,both of which were at a high level.However,the nature of the generated restorative dentin and the state of dental pulp required more study by histological observation.