活性云母组合物液抗鸡柔嫩艾美尔球虫感染的效果

Effects of an active liquid compound containing mica on an Eimeria tenellainfection in chickens

目的观察活性云母组合物液对柔嫩艾美尔球虫(E.tenella)感染雏鸡的防治效果。方法 7日龄雏鸡200只,随机分为10组(每组20只),分别为A组(空白对照组,不感染球虫,不用活性云母组合物液组)、B组(阳性对照组,即感染球虫,不用活性云母组合物液组)、C组(地克珠利预防组)、D组(6%活性云母组合物液预防组)、E组(4%活性云母组合物液预防组)、F组(2%活性云母组合物液预防组)、G组(地克珠利治疗组)、H(6%活性云母组合物液治疗组)、I组(4%活性云母组合物液治疗组)、J组(2%活性云母组合物液治疗组)。C、D、E、F组分别于8日龄起通过饮水给予地克珠利和不同浓度的活性云母组合物液,连续7 d。14日龄时除空白对照组外其余各组分别经口感染柔嫩艾美耳球虫孢子化卵囊5×104个/只。感染球虫卵囊后,C、D、E、F组饮用水换成不含地克珠利和活性云母组合物液的水;G、H、I、J组分别通过饮水给予地克珠利和不同浓度活性云母组合物液,保证饮水充足,连续7 d。所有雏鸡自由采食和饮水。攻虫后第8 d剖杀,通过平均增重、相对增重率、盲肠病变、卵囊排出情况计算抗球虫指数(Anticoccidial index, ACI)结果 D组的平均增重与对照组B组的平均增重进行两两相比差异有统计学意义(t=-46.0341,P<0.05),相对增重率为76.73%;E组的平均增重与对照组B组的平均增重进行两两相比差异有统计学意义(t=-77.9200,P<0.05),相对增重率为72.97%;C组和G组的平均增重均显著高于对照组B组的平均增重(t1=-147.9948,t2=-40.2998,P<0.05),相对增重率分别为86.48%和83.99%。D组卵囊减少率为93.4%,E组为91.3%,高于I组和J组。综合抗球虫指数显示D组和E组预防柔嫩艾美尔球虫感染效果较好,其抗球虫指数ACI分别为171.7和166.0;其次为H组和I组抗柔嫩艾美尔球虫感染效果较好,其抗球虫指数ACI分别为163.6和161.7;其余两组ACI值均小于160,抗球虫效果一般。结论 6%活性云母组合物液对鸡E.tenella感染具有较好的预防效果,而治疗效果一般。

Objective To observe the efficacy with which an active liquid compound containing mica controlled Eimeria tenellain chickens. Methods Two hundreds seven-day-old chicks were randomly divided into ten groups:group A(blank control group,unchallenged with E.tenella oocysts and untreated with an active liquid compound containing mica),group B(positive control group,challenged with E.tenella oocysts but untreated with an active liquid compound containing mica),group C(group prophylactically treated with diclazuril),group D(group prophylactically treated with a 6% active liquid compound containing mica),group E(group prophylactically treated with a 4%active liquid compound containing mica),group F(group prophylactically treated with a 2%active liquid compound containing mica),group G(group prophylactically treated with diclazuril),H(group treated with a 6% active liquid compound containing mica),group I(group treated with a 4% active liquid compound containing mica),and group J(group treated with a 2%active liquid compound containing mica).Groups C,D,E,and F were given diclazuril and different concentrations of the active liquid compound containing mica in drinking water starting at 8 dof age for 7 dconsecutively.All groups except those in Group A were orally challenged with 5×104 E.tenella sporulated oocysts at 14 dof age.After infection with coccidian oocysts,the drinking water of groups C,D,E,and F was changed to water that did not contain diclazuril and the active liquid compound containing mica.Groups G,H,I,and J were given diclazuril and different concentrations of the active liquid compound containing mica via drinking water for 7 dconsecutively.Sufficient drinking water was continuously supplied,and all chicks had access to feed and water ad libitum.On day 8 after the challenge,the anticoccidial index(ACI)was calculated based on the average weight gain,the relative rate of weight gain,cecal lesions,and oocyst discharge. Results The average weight gain of group D and the control group(group B)differed significantly(t=-46.0341,P<0.05),and the relative rate of weight gain was 76.73%.The average weight gain of group E differed significantly from that of the control group B(t=-77.9200,P<0.05),and the relative rate of weight gain was 72.97%.The average weight gain of groups C and G was significantly greater than that of control group B(t1=-147.9948,t2=-40.2998,P<0.05),and the relative rate of weight gain was 86.48% and 83.99%,respectively.The rate of reduction in oocysts in groups D and E was 93.4%and 91.3%,respectively,and those rates were higher than that in groups I and J.The overall ACI indicated that an E.tenellainfection was prevented more effectively in groups D and E,and their ACI was 171.7 and166.0,respectively.Groups H and I had the next highest ACI,which was 163.6 and 161.7,respectively.The ACI in the other two groups was less than 160,and anti-coccidial action was average. Conclusion The 6%active liquid compound containing mica was effective at preventing an E.tenella infection in chicks but its therapeutic efficacy in infected chicks was average.