泉州地区耐大环内酯类肺炎链球菌血清型及多位点序列分型研究

Serotype analysis and multi-locus sequence typing of macrolide-resistant Streptococcus pneumoniae in the Quanzhou Region

目的了解泉州地区2013-2016临床分离的耐大环内酯类肺炎链球菌(MRSP)血清型及多位点序列分型。方法收集三家医院2013-2016年临床分离的MRSP 101株,采用多重PCR进行血清型鉴定,采用多位点序列分型(MLST)技术分析菌株序列型(ST)。结果血清型分析显示,101株MRSP以19F(62.4%)、6B(15.8%)、23F(10.9%)血清型为主,占89.1%(90/101)。MLST分析101株MRSP共分为35种STs,主要存在ST271(33.7%,34/101)、ST320(17.8%,18/101)和ST81(5.9%,6/101) 3个优势型别,其中ST271以19F血清型为主。该地区MRSP主要流行的克隆复合体为CC320/271。新发现6个STs(ST7181、ST10228、ST12112、ST12113、ST12114、ST12115)并录入MLST数据库。结论泉州地区MRSP的ST型别具有多样性,但主要流行的克隆复合体为CC320/271,19F、23F、6B是主要流行血清型,其中19F血清型以ST271和ST320为主。

Objective To ascertain the serotypes and to multi-locus sequence type macrolide-resistant Streptococcus pneumoniae(MRSP) isolated in the Quanzhou district from 2013 to 2016. Methods One hundred and one strains of MRSP were isolated from Quanzhou Women’s and Children’s Hospital, the Second Hospital Affiliated with Fujian Medical University, and the First Hospital Affiliated with Fujian Medical University in Quanzhou. Serotypes were analyzed using multiplex PCR, and STs were studied using MLST. Results The major serotypes of the 101 MRSP strains were 19 F(62.4%), 6 B(15.8%), and 23 F(10.9%), accounting for 89.1%(90/101) of the strains. MLST analysis indicated that the 101 MRSP strains were divided into 35 STs, and there were three main types: ST271(33.7%, 34/101), ST320(17.8%, 18/101), and ST81(5.9%, 6/101). The 19 F serotype was the main ST in ST271. The main clonal complex of MRSP was CC320/271 in Quanzhou. Six new STs, namely ST7181, ST10228, ST12112, ST12113, ST12114, and ST12115, have been entered in the MLST database. Conclusion The STs of MRSP in the Quanzhou region were diverse, but the main clone complex was CC320/271. 19 F, 23 F, and 6 B were the prevalent serotypes, and ST271 and ST320 were the prevalent types of 19 F.