808nm激光介导的光生物调节对高糖环境下成纤维细胞活性氧稳态的影响

Effect of Photobiomodulation Mediated by 808 nm Laser on Active Oxygen Steady-State in CCC-ESFs under High Glucose Environment

创面愈合延迟是最具挑战性的糖尿病临床并发症之一,其与活性氧(ROS)的过量生成有关。光生物调节作用(PBM)可以从多方面促进创面愈合,可作为糖尿病患者创面延迟愈合的一种治疗方法。本文研究了PBM对高糖培养的人胚胎皮肤成纤维细胞(CCC-ESFs)活性氧稳态的影响,探讨了PBM对改善细胞氧化应激损伤的作用。首先体外培养CCC-ESFs,细胞被随机分为对照组(正常培养基和高糖培养基)和808 nm激光照射组(功率密度分别为10,20,40 mW/cm2,能量密度分别为1.5,3,6,12 J/cm2),对照组不照光。高糖造模48 h后进行激光照射,随后分别检测细胞的增殖活力、ROS含量、总超氧化物歧化酶含量、总抗氧化能力、线粒体膜电位和相关细胞因子表达量。结果表明:高糖环境降低了细胞的增殖活力,ROS含量显著增多,抗氧化能力下降,细胞凋亡增多;采用808 nm激光照射后,细胞的增殖效应没有得到明显改善,但细胞的ROS含量下降,抗氧化酶表达量出现上升趋势,促炎细胞因子表达量降低。这些结果表明,PBM可以修复高糖环境下产生的氧化应激损伤,调节炎症反应,促进创伤愈合。

Delayed wound healing is one of the most challenging clinical complications of diabetes mellitus(DM)and is related to the excessive production of reactive oxygen species(ROS).Photobiomodulation(PBM)can promote the healing of delayed wounds in DM patients.We studied the effect of PBM on the active oxygen homeostasis of human embryonic skin fibroblasts(CCC-ESFs)that were cultured in a high glucose environment,and explored the effect of PBM on improving oxidative stress injuries.CCC-ESFs were cultured in vitro,and the cells were randomly divided into control group(normal glucose group and high glucose group)and 808 nm laser irradiation group(power density:10,20,and 40 mW/cm2;energy density:1.5,3,6,and 12 J/cm2).After 48 hours of high glucose modeling,the experimental group was irradiated with a laser and we measured the cell proliferation,ROS content,total superoxide dismutase,total antioxidant capacity,mitochondrial membrane potential and the expression level of the related cytokines.The results show that cell proliferation activity was reduced,ROS content was significantly increased,antioxidant capacity was decreased and cell apoptosis was increased in the high glucose environment;however,the proliferation effect of the cells was not significantly improved after 808 nm laser irradiation,but ROS content of the cells decreased,the expression of antioxidant enzymes showed an upward trend,and the expression of pro-inflammatory cytokines decreased.These results show that PBM can repair oxidative stress damage,regulate inflammatory responses,and promote wound healing under a high glucose environment.