免标记型免疫传感器的构建及其对毛织品微痕迹的检测

The development of a label-free immunosensor and its detection of micro traces of wool textiles

为检测毛织品微痕迹,设计了一种针对羊毛角蛋白的免标记型免疫传感器。通过层层自组装技术构建免疫传感器,用3-巯基丙酸(MPA)在电极表面形成单分子层,活化羧基端后与抗体结合,利用免疫反应检测羊毛角蛋白。选用5种抗原分析了免疫传感器的特异性,利用不同浓度的羊毛角蛋白溶液分析了免疫传感器的敏感性。结果显示:免疫传感器对于羊毛角蛋白具有选择性,并且在检测羊毛角蛋白时表现出了优异的灵敏度。免疫传感器的线性检测范围为0.10~100.00μg/mL,最低检测限可以达到11.53 ng/mL。研究结果表明,该免疫传感器可以用于毛织品微痕迹的鉴定分析和定量计算,并且对其具有极高的检测灵敏性。毛织品的电化学免疫检测体系的建立,为毛织品的超灵敏检测提供了一种新的思路,拓展了毛织品文物的分析方法。

In order to detect micro traces of wool textiles,the author designed a label-free immunosensor for wool keratin.An immunosensor was constructed by layer-by-layer self-assembly technique.3-mercaptopropionic acid(MPA)was used to form a monomolecular layer on the electrode surface.After activating the carboxyl terminal,it was bound to the antibody.The wool keratin was detected by immunoreaction.Five antigens were selected to analyze the specificity of the immunosensor,and different concentrations of wool keratin solutions were employed to analyze the sensitivity of the immunosensor.The results showed that the immunosensor was selective for wool keratin and showed excellent sensitivity when detecting wool keratin.The linear detection range of the immunosensor was 0.10~100.00μg/mL,and the limit of detection(LOD)was up to 11.53 ng/mL.The results confirm that the proposed immunosensor can be used for identification and quantitative calculation of micro traces of wool textiles,and has extremely high sensitivity for micro traces.The establishment of electrochemical immunoassay system for wool textiles provides a new idea for the ultra-sensitive detection of micro traces of wool textiles,and expands the analysis methods of wool textile cultural relics.