低纬高原甘蔗白叶病植原体传播方式测定分析

Determination and analysis of the transmission mode of sugarcane white leaf phytoplasma in low latitude plateau

甘蔗白叶病(Sugarcane white leaf,SCWL)是由16SrXI组植原体引起的甘蔗重要病害[1],主要通过带病蔗种传播,还可在田间通过叶蝉自然传播[2,3]。该病1954年首次在泰国发现[4],现已在印度、巴基斯坦、斯里兰卡、菲律宾、缅甸、越南等国普遍发生,给当地蔗糖业造成巨大经济损失[5,6]。

Sugarcane white leaf(SCWL)is a new destructing disease of sugarcane that has rapidly expanded in low latitude plateau of Yunnan in recent years,and it is extremely harmful to sugarcane production.In order to study and clarify the transmission mode of SCWL phytoplasma in low latitude plateau,the samples were collected from Gengma sugarcane-producing area of Lincang City,Yunnan Province.This area had the most severe SCWL disease from 2018 to 2019.The nested-PCR results showed that five susceptible mainly sugarcane varieties grown from diseased-seedcane were all infected in five treatments of new planting and ratoon sugarcane,and the disease incidence were 11.7%to 72.9%.While the disease incidence in plants grown from disease-free seedcane was 0%.Moreover,the disease incidence in plants grown from disease-free seedcane+insect-proof netting was also 0%.It observed that the primary transmission mode of SCWL phytoplasma was infected seedcane in Gengma sugarcane area(having serious disease prevalence)in Lincang City,Yunnan Province,and there was no insect vector transmission.The results of this study enriched the relevant theoretical and technical foundations of sugarcane phytoplasma disease,and provided theoretical guidance and scientific basis for the effective prevention and control of SCWL in low-latitude plateaus.