文冠果油对SD孕鼠毒性和胎鼠致畸性研究

Toxicity on SD pregnant rats and teratogenicity in fetal rats fed by Xanthoceras sorbifolium Bunge.seed oil

研究文冠果油对SD孕鼠的毒性和胎鼠致畸性。以花生油为阴性对照,环磷酰胺为阳性对照,文冠果油0.92、1.83、3.67 g/kg为低、中、高剂量组,灌胃SD受孕大鼠,解剖观察孕鼠胚胎毒性指标和胎鼠生长发育指标,分析文冠果油对胎鼠外观畸形、骨骼发育和内脏、脑系统发育的影响。结果表明:文冠果油各剂量组孕鼠饮食、活动正常,未出现中毒特征。与阴性对照组相比,各剂量组孕鼠的体重没有受到影响,体重增重、子宫连胎重、体重净增重、黄体数、着床数、活胎数、吸收胎数、死胎数均无显著性差异(P>0.05);各剂量组胎鼠的体重、体长、外观畸形率、骨骼发育畸形率和内脏、脑系统发育畸形率也没有显著性差异(P>0.05)。文冠果油未观察到有害作用剂量(NOAEL)为3.67 g/kg,对SD大鼠未见明显母体毒性、胚胎毒性和致畸性。

The toxicity on SD pregnant rats and teratogenicity in fetal rats of Xanthoceras sorbifolium Bunge.seed oil were studied.With peanut oil as negative control and cyclophosphamide as positive control,0.92,1.83 g/kg and 3.67 g/kg of X.sorbifolium Bunge.seed oils were used as the low,middle and high groups to administrate SD pregnant rats,and the pregnant rats embryo toxicity indexes and fetal rats growth and development indexes were observed by anatomy,and the effects of X.sorbifolium Bunge.seed oil on the appearance deformity,bone development and internal organ and brain development of fetal rats were analyzed.The results showed that the diet and activity of pregnant rats in each dose group of X.sorbifolium Bunge.seed oil were normal,and no poisoning characteristics appeared.The weight of pregnant rats in each dose group was not affected.Compared with the negative control group,the weight gain,uterine continuous fetal weight,net weight gain,corpus luteum number,implantation number,live fetus number,absorbed fetus number and stillbirths number of pregnant rats in each dose group had no significant difference(P>0.05).There was no significant difference in the weight,body length,appearance deformity rate,bone development deformity rate and visceral and brain development deformity rate of fetal rats in each dose group(P>0.05).No harmful effects(NOAEL)of X.sorbifolium Bunge.seed oil was observed at 3.67 g/kg,and there was no obvious maternal toxicity,embryo toxicity and teratogenicity to SD rats.