再生水灌溉对5种常见草坪草种子萌发的影响及作用机理

Effects of Reclaimed Water Irrigation on Seed Germination of Five Common Turfgrass Varieties and Its Mechanism

以结缕草(Zoysia japonica Steud)、高羊茅(Festuca elata Keng ex E.Alexeev)、黑麦草(Lolium perenne L.)、狗牙根(Cynodon dactylon(L.)Pers.)和早熟禾(Poa annua L.)为试验材料,设置30%(T 1)、60%(T_(2))和100%(T_(3))等3个再生水灌溉浓度,以清水灌溉为对照(ck),测定了不同浓度再生水灌溉对种子萌发和萌发过程中活性氧代谢及激素含量的影响。结果表明,再生水灌溉对草坪草种子萌发具有一定的抑制作用。随着再生水浓度的升高,草坪草种子发芽率、发芽势、发芽指数及赤霉素(GA_(3))含量均逐渐降低,而H_(2)O_(2)含量、O_(2)^(-)·产生速率、丙二醛(MDA)含量、相对电导率及脱落酸(ABA)含量均逐渐上升;超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX)活性均随再生水浓度的升高表现为先升高后降低的趋势,以60%浓度为分界线。5种草坪草种子对再生水的敏感性存在明显差异,高羊茅和狗牙根对再生水耐受性明显高于结缕草、黑麦草和早熟禾,主要由抗氧化能力和激素含量差异造成。

In this study,the Zoysia japonica,Festuca elata,Lolium perenne,Cynodon dactylon and Poa annua were selected as experimental materials to determine the effects of different concentrations of reclaimed water irrigation on seed germination and the metabolism of reactive oxygen species and the content of hormones during germination.During the process of experiment,3 irrigation concentrations of reclaimed water,namely 30%(T 1),60%(T_(2))and 100%(T_(3))were set,and the water irrigation as the control.The results showed that reclaimed water irrigation could inhibit the germination of turf grass seeds to some extent.With the increase of reclaimed water concentration,the germination percentage,germination potential,germination index and gibberellin(GA_(3))content of grass seeds decreased gradually,while the H_(2)O_(2) content,O_(2)^(-)·production rate,malondialdehyde(MDA)content,relative electrical conductivity and abscisic acid(ABA)content increased gradually.The activities of superoxide dismutase(SOD),peroxidase(POD),catalase(CAT)and ascorbate peroxidase(APX)were firstly increased and then decreased with the increase of reclaimed water concentration,with 60%concentration as the dividing line.There were significant differences in the sensitivity of 5 turfgrass seeds to reclaimed water,and the tolerance of F.elata and C.dactylon roots to reclaimed water was significantly higher than that of Z.japonica,L.perenne and P.annua,which was mainly caused by the differences in antioxidant capacity and hormone content.