杜鹃兰类原球茎快速增殖研究

Study on Rapid Proliferation of Protocorm-like Bodies in Cremastra appendiculata

以杜鹃兰类原球茎为材料,研究基本培养基、不同激素配比和绘制类原球茎增殖生长曲线,获得类原球茎增殖的最适条件。结果表明,杜鹃兰类原球茎快速增殖最佳培养方案是基本培养基为B 5,转接入B 5+6-BA 0.3 mg·L^(-1)+NAA 1.0 mg·L^(-1)+IBA 0.5 mg·L^(-1)+IAA 0.3 mg·L^(-1)+活性炭1.0 g·L^(-1)+土豆泥30.0 g·L^(-1)培养基中,经过45 d培养后,使杜鹃兰类原球茎增殖倍数最高达8.20倍。成功建立起杜鹃兰类原球茎稳定快速增殖的体系。

In this study,the optimal conditions for proliferation of protocorm-like bodies in Cremastra appendiculata were obtained by studying the basic medium,different hormone ratio and drawing the growth curve of procorm.The results showed that B 5 was the best basic medium for rapid proliferation of C.appendiculata protocorm-like bodies.When the protocorm-like bodies were transferred the into B 5+6-BA 0.3 mg·L^(-1)+NAA 1.0 mg·L^(-1)+IBA 0.5 mg·L^(-1)+IAA 0.3 mg·L^(-1)+activated carbon 1.0 g·L^(-1)+mashed potatoes 30.0 g·L^(-1),and after 45 d of culture,the maximum multiplication ratio of C.appendiculata protocorm-like bodies was 8.20 folds.In this study,a stable and rapid proliferation system of C.appendiculata protocorm-like bodies was successfully established.