摘要
试验旨在建立检测饲料中16种氨基酸的液相色谱-串联质谱(LC-MS)方法。采用C18反相色谱柱进行组分分离,以0.1%甲酸水和甲醇按梯度洗脱程序进行洗脱,优化色谱条件和质谱参数。结果表明,16种氨基酸在0.01~50.00μmol/L范围内线性良好,线性相关系数均大于0.9910,检出限为1~10 ng/g,定性重复性为0.02%~0.12%,定量重复性为0.11%~0.94%,回收率为97.3%~104.0%。在不同种类的样品测试中,该方法与国家标准方法的相对偏差均小于5%,结果准确可靠。试验方法检测效率高、灵敏度高、回收效果良好、定性和定量准确,能够满足饲料中16种氨基酸的常规检测。
The purpose of the experiment was to establish a liquid chromatography-tandem mass spectrometry(LC-MS)method for the detection of 16 amino acids in feed.A C18 reversed-phase chromatographic column was used for component separation,and elution was carried out with 0.1%formic acid water and methanol according to a gradient elution procedure,and the chromatographic conditions and mass spectrometry parameters were optimized.The results showed that the 16 amino acids had good linearity in the range of 0.01~50.00μmol/L,the linear correlation coefficients were all greater than 0.9910,the detection limit was 1~10 ng/g,and the qualitative repeatability was 0.02%~0.12%.The quantitative repeatability was 0.11%~0.94%,and the recovery rate was 97.3%~104.0%.In different types of sample tests,the relative deviation between this method and the national standard method is less than 5%,and the results are accurate and reliable.The method can improve detection efficiency,high sensitivity,good recovery effect,qualitative and quantitative accuracy,and can meet the routine detection of 16 amino acids in feed.
作者
贺习文
雷浩
高勤叶
赵雪宁
温卉
马川
He Xiwen;Lei Hao;Gao Qinye;Zhao Xuening;Wen Hui;Ma Chuan(Shaanxi QinYun Agricultural Products Inspection and Testing Co.,Ltd,Shaanxi Weinan 714000)
出处
《现代畜牧兽医》
2021年第6期27-30,共4页
Modern Journal of Animal Husbandry and Veterinary Medicine
关键词
液相色谱-串联质谱法
氨基酸
饲料
水解
Liquid chromatography-tandem mass spectrometry
Amino acid
Feed
Hydrolysis