HPLC法测定安神补脑颗粒中维生素B_(1)的含量

Determination of Vitamin B_(1) in Anshen Bunao Granules by HPLC

目的建立测定安神补脑颗粒中维生素B 1含量的高效液相色谱(HPLC)法。方法采用C 18色谱柱(4.6×250 mm,5μm),甲醇-乙腈-0.02 mol·L^(-1)庚烷磺酸钠溶液(含1%三乙胺,用磷酸调节pH值至5.5)(9∶9∶82)为流动相;检测波长为254 nm。柱温:40℃;进样量:10μL;流速:1.0 mL·min^(-1)。结果维生素B 1在0.0125~0.1000 mg·mL^(-1)浓度范围线性良好(r=0.9998),平均回收率为99.88%,RSD=0.58%(n=9)。结论该方法简便、重复性好、准确,可作为安神补脑颗粒质量控制。

OBJECTIVE To establish a HPLC method for the determination of Vitamin B 1 in Anshen Bunao Granules.METHODS C 18 column(4.6×250 mm,5μm)was used.The mobile phase was methanol-acetonitrile-0.02 mol·L^(-1)sodium heptanesulfonate solution(containing 1%triethylamine,pH adjusted to 5.5 with phosphoric acid solution)(9∶9∶82).The detection wavelength was 254 nm.The column temperature was 40℃.The injection volume was 10μL.The flow rate was 1.0 mL·min^(-1).RESULTS The calibration curves was linear in the concentration range of 0.0125-0.1000 mg·mL-1(r=0.9998),the average recovery was 99.88%,RSD=0.58%(n=9).CONCLUSION The method is simple,reliable and accurate,which is suitable for the quality control of Anshen Bunao Granules.