基于网络药理学探讨澳洲茄碱抗肿瘤机制

The underlying anti-cancer mechanism of Solasonine:A network pharmacology analysis

目的基于网络药理学,构建澳洲茄碱抗肿瘤的“靶点-通路”网络,探讨澳洲茄碱抗肿瘤的作用机制。方法通过TCMSP及Pharm Mapper收集澳洲茄碱潜在靶点,OMIM及Genecards数据库收集肿瘤疾病靶点,将澳洲茄碱潜在靶点与肿瘤疾病靶点交集,获得澳洲茄碱-肿瘤共同靶点。运用String数据库结合Cytoscape 3.7.2绘制澳洲茄碱-肿瘤共同靶点的蛋白相互作用网络图(PPI),筛选核心靶点。最后,基于R3.6.0软件用Bioconductor生物信息软件包,对澳洲茄碱-肿瘤共同靶点进行进行GO功能与KEGG通路富集分析。结果澳洲茄碱-肿瘤共同靶点79个,核心基因3个:IGF1R、MAP2K1、CHEK1。通路分析显示澳洲茄碱参与多条肿瘤相关通路的调控,主要涉及PI3K-Akt信号通路、MAPK信号通路、Ras信号通路等经典肿瘤信号通路。结论澳洲茄碱调控多个靶点,同一个靶点参与多个生物学过程和信号通路,澳洲茄碱可能通过多靶点、多通路发挥抗肿瘤作用。

Objective To construct a"target-pathway"based on network pharmacology for the exploration of the anti-cancer mechanism of Solasonine.Methods Potential anti-cancer targets of Solasonine were searched through the TCMSP and Pharm Mapper databases,while the cancer unique targets were searched through OMIM and Genecards databases.Any targets in common of both were obtained.The protein-protein interaction(PPI)network of targets was built using the STRING database and Cytoscape 3.7.2 software.The core targets of PPI network were further identified.Gene ontology and KEGG pathway enrichment were analyzed with Bioconductor package for R3.6.0.Results A total of 79 common targets of Solasonine-cancer and 3 core genes(IGF1R,MAP2K1 and CHEK1)were identified.Pathway analysis revealed that Solasonine was involved in the regulation of multiple cancer-related pathways,including PI3K-Akt signaling,MAPK signaling and Ras signaling pathways.Conclusion Solanine has multiple anti-cancer targeting properties involving in multiple biological processes and signaling pathways,which contribute to its anti-cancer activities.