太子参NF-Y基因家族鉴定及表达分析

Identification and Expression Analysis of NF-Y Gene Family in Pseudostellaria heterophylla

核因子Y(NF-Y)是真核生物中一种重要的转录因子.采用生物信息学方法从太子参转录组数据库中鉴定NF-Y蛋白家族成员,通过转录组数据分析各基因在不同组织中的表达水平,研究NF-Y基因对土壤水分及蔗糖信号的响应.通过对3个转录组数据库的检索,确定了太子参NF-Y蛋白家族的3个亚家族,包含9个PhNF-YA成员,10个PhNF-YB成员及5个PhNF-YC成员,发现PhNF-YA4等9个成员在叶中有较高表达,PhNF-YA3在块根皮部及PhNF-YB3和PhNF-YB8在块根木质部中有较高表达.干旱胁迫可显著上调11个成员的表达,2个成员的表达量随土壤水分含量的上升表现出上调的趋势.大部分基因能够响应蔗糖浓度的变化.这些结果暗示了水分、蔗糖等因子可通过影响器官中NF-Y蛋白的表达,改变植物NF-Y蛋白间的相互作用关系,进而调控器官的形态建成及逆境响应.

Nuclear factor Y(NF-Y)is an important transcription factor in eukaryotes.In this study,bioinformatics was used to identify NF-Y protein family members from the transcriptome database of P.heterophylla,and the transcriptome data were used to analyze the expression level of each gene in different tissues.Their response to soil water stress and sucrose was also studied.Three subfamilies of NF-Y protein family of P.heterophylla were identified by searching three transcriptome databases,including 9 members of PhNF-YA,10 members of PhNF-YB and 5 members of PhNF-YC.It was found that 9 members,such as PhNF-YA4,were highly expressed in leaves.High expression level of PhNF-YA3 and PhNF-YB3,PhNF-YB8 were observed in bark and xylem of tuberous root,respectively.The expression of 11 members in the tuberous root of P.heterophylla was significantly up-regulated under drought stress.Two members had up-regulated expression in microtuberous root along with the increase of soil water content.Most genes could respond to content change of sucrose.These results suggest that some factors can affect the expression level of NF-Y protein in organs,change the interaction between different NF-Y proteins in plants,and regulate the morphogenesis of organs and response to stresses.