Ag2S@BSA存储液对纯钛表面成骨细胞行为的影响

Effects of a AgS@BSA-containing storage solution on osteoblasts behaviors on pure titanium surface

目的:研究含牛血清白蛋白(bovine serum albumin,BSA)包裹的硫化银存储液对纯钛表面特性及成骨细胞生物学行为的影响。方法:纯钛试件经打磨清洗后分为4组,分别置于空气、生理盐水、BSA溶液、牛血清白蛋白包裹的硫化银溶液(BSAcoated Ag2S solution,Ag2S@BSA)中保存2周后,采用扫描电镜、X线能谱仪和光学接触角仪分析纯钛表面特性的变化;将MC3T3-E1成骨细胞接种于各组钛片表面,观察成骨细胞的黏附、增殖和分化行为。结果:扫描电镜和能谱分析结果显示,各组钛表面微形貌无明显差异,空气存储后未见明显改变,经生理盐水存储后钛表面散在分布氯化钠晶体,经BSA溶液存储后含碳有机物增加,经Ag2S@BSA存储后钛表面均匀吸附含硫和银元素的纳米颗粒。与其余3种存储方式相比,Ag2S@BSA溶液存储可显著减小钛表面水接触角,增大表面能,显著促进成骨细胞的黏附、增殖和成骨功能蛋白表达。结论:Ag2S@BSA存储液能优化钛表面特性,增强其成骨活性。

Objective:This study aims to investigate the effects of bovine serum albumin(BSA)coated Ag2S storage solution on the surface characteristics of pure titanium and the behaviors of osteoblasts.Methods:After polishing and cleaning,pure titanium disks were stored in air,saline,BSA solution and BSA-coated Ag2S storage solution(Ag2S@BSA)respectively for two weeks.Scanning electron microscope(SEM),energy dispersive spectrometer(EDS)and contact angle test were used to analyze surface characteristics of pure titanium preserved in four media.Assays of adhesion,proliferation,and differentiation of MC3T3-E1 cells were conducted to evaluate the biocompatibility of different samples.Results:SEM and EDS results demonstrated that sodium chloride crystals were scattered on titanium surface in the saline group,while carbon and oxygen distribution were denser in the BSA group.Nano-sized particles containing silver and sulfur were adsorbed on the titanium surface without changing the original morphology.Compared with the other three groups,titanium surfaces preserved in Ag2S@BSA solution showed superior hydrophilicity and significantly promoted the adhesion proliferation and differentiation abilities of osteoblasts with high expression of osteogenesis-related proteins.Conclusion:The Ag2S@BSA-containing storage solution can enhance the osteogenic activity of osteoblasts by improving the surface characteristics of pure titanium.