黄芪注射液对肺癌细胞凋亡及PI3K/AKT信号通路的影响

Effect of Radix Astragali Injection on Apoptosis of Lung Cancer Cells and PI3K/AKT Signaling Pathway

目的:探讨黄芪注射液对肺癌细胞凋亡及磷脂酰肌醇3-激酶/蛋白激酶B(PI3K/AKT)信号通路的影响。方法:取对数生长期、状态良好的A549细胞,随机分为对照组、实验1组、实验2组。对照组用常规培养基培养干预,在此基础上,实验1组用100 mg/mL的黄芪注射液干预,实验2组用200 mg/mL的黄芪注射液干预。采用MTT法检测细胞增殖,双染法检测细胞凋亡指数,Transwell实验检测细胞迁移,Western-blot检测PI3K/AKT蛋白表达,流式细胞仪检测细胞周期。结果:处理24 h、48 h后,与对照组比较,实验1组与实验2组细胞增殖指数、迁移距离及PI3K、AKT蛋白相对表达水平降低(P<0.05),细胞凋亡指数升高(P<0.05);与实验1组比较,实验2组细胞增殖指数、迁移距离及PI3K、AKT蛋白相对表达水平更低(P<0.05),细胞凋亡指数更高(P<0.05)。处理48 h后,与对照组比较,实验1组与实验2组GO/G1期比例降低(P<0.05),S期、G2/M期比例升高(P<0.05);与实验1组比较,实验2组GO/G1期比例更低(P<0.05),S期、G2/M期比例更高(P<0.05)。结论:黄芪注射液能抑制肺癌细胞PI3K/AKT信号通路的激活,影响细胞正常周期,从而促进细胞凋亡,抑制细胞增殖与迁移。

Objective:To discuss the effect of Radix Astragali injection on apoptosis of lung cancer cells and phosphatidylinositol-3-kinase/protein kinase B(PI3K/AKT)signaling pathway.Methods:A549 cells at logarithmic growth phase and in a good condition were selected and randomly divided into the control group,the Experiment Group 1,and the Experiment Group 2.The control group was cultured in a routine medium for intervention;the Experiment Group 1 was intervened with 100 mg/mL Radix Astragali injection based on the intervention of the control group;the Experiment Group 2 was intervened with 200 mg/mL Radix Astragali injection based on the intervention of the control group.MTT method was used to detect cell proliferation.Double staining method was used to detect apoptosis index.Transwell assay was used to detect cell migration.Western-blot method was used to detect the expression of protein of PI3K/AKT.Flow cytometry was used to detect cell cycle.Results:After intervention for 24 h and 48 h,in the Experiment Group 1 and the Experiment Group 2,indexes of cell proliferation,migration distances,and the relative expression levels of protein of PI3K and AKT were decreased when compared with those in the control group(P<0.05),and apoptosis indexes were increased(P<0.05).After intervention for 24 h and 48 h,in the Experiment Group 2,index of cell proliferation,migration distance,and the relative expression levels of protein of PI3K and AKT were lower than those in the Experiment Group 1(P<0.05),and apoptosis index was higher than that in the Experiment Group 1(P<0.05).After intervention for 48 h,in the Experiment Group 1 and the Experiment Group 2,the proportions of G0/G1 phase were decreased when compared with those in the control group(P<0.05),and the proportions of S phase and G2/M phase were increased(P<0.05).After intervention for 48 h,in the Experiment Group 2,the proportion of G0/G1 phase was smaller than that in the Experiment Group 1(P<0.05),and the proportions of S phase and G2/M phases were larger than those in the Experiment Group 2(P<0.05).Conclusion:Radix Astragali injection can inhibit the activation of PI3K/AKT signaling pathway in lung cancer cells and have an effect on normal cell cycle,thus promoting apoptosis and inhibiting proliferation and migration of cells.