川产橘核传统与新兴药用品种的等同性分析

Analysis of Equivalence Between Traditional and Nontraditional Medicinal Varieties of Citri Reticulatae Semen from Sichuan Province

目的:研究川产传统药用橘核与新兴药用橘核在亲缘关系、形状大小和黄酮类化合物含量上的差异,探讨二者的等同性。方法:收集6份川产传统药用橘核(大红袍)和23份川产新兴药用橘核,采用相关序列扩增多态性分子标记(SRAP)研究橘核样品间的亲缘关系;使用体视显微镜观测样品的形状大小;借助高效液相色谱法(HPLC)检测柚皮苷、橙皮苷和新橙皮苷的含量,用SIMCA 14.1软件分别对形状、大小和黄酮类化合物含量进行聚类分析,研究传统药用橘核和新兴药用橘核在性状大小和化学成分上的相似度。结果:SRAP亲缘关系分析能有效区分不同品种的橘核样品,部分产地相同或相邻的不同品种的橘核亲缘关系较近,还发现亲缘关系较近的不同品种橘核在形状大小和黄酮类化合物含量上相似度也高,但总体看来传统药用品种橘核与大部分新兴药用品种区别较大。结论:通过分析传统药用品种和非传统药用品种在遗传物质、外观性状和有效成分含量的差异,发现部分地区的椪柑与传统药用品种大红袍等同性最大,有作为新兴药用品种的潜力。

Objective:To study the differences in genetic relationship,shape,size,and flavonoid content between traditional and nontraditional medicinal varieties of Citri Reticulatae Semen produced in Sichuan province as well as their equivalence.Method:Six batches of traditional medicinal Citri Reticulatae Semen(Citrus reticulata’Dahongpao’)and 23 batches of nontraditional medicinal varieties were collected,and their genetic relationship was explored using sequence-related amplified polymorphism(SRAP)markers.Following the observation of their shapes and sizes under a stereomicroscope,the contents of naringin,hesperidin,and neohesperidin were measured by high performance liquid chromatography(HPLC).SIMCA 14.1 software was used for cluster analysis of their shapes,sizes,and flavonoid contents,thus figuring out the similarities between the traditional and nontraditional medicinal varieties in character,size,and chemical components.Result:SRAP markers-based genetic relationship analysis effectively distinguished different Citri Reticulatae Semen varieties from each other.Some samples collected from the same or adjacent places exhibited a close genetic relationship and they shared high similarities in shape,size,and flavonoid content.However,the traditional medicinal Citri Reticulatae Semen was still quite different from most nontraditional medicinal varieties.Conclusion:The analysis of differences in genetic materials,appearance,character,and active ingredient content between the traditional and nontraditional medicinal varieties revealed that the equivalence of C.reticulata’Ponkan’samples from some regions with the traditional medicinal variety was the largest,enabling them to be considered as the emerging medicinal variety.