采用傅里叶红外光谱技术快速分析碳青霉烯耐药大肠埃希菌同源性

Rapid identification of homology of carbapenem-resistant Escherichia coli by FTIR

目的:评估傅里叶红外光谱技术(FTIR)对临床分离的碳青霉烯耐药大肠埃希菌(CREC)同源性分析的能力。方法:26株CREC为2018年从来自全国9个省、市(湖南、吉林、浙江、福建、上海、安徽、重庆、云南、河南)的样本中分离,FTIR检测菌株在900~1200 cm^(-1)区域的红外吸收光谱,使用欧式距离和平均连接聚类方法进行多变量分析;并对菌株进行全基因组测序(WGS)分析单核苷酸多态性(SNP)。结果:FTIR将26株CREC分为14个红外光谱(IR)型,且相同IR型的菌株均属于同一序列(ST)型。相比WGS对CREC的聚类分析,FTIR的聚类分析结果一致性为92.3%(24/26)。结论:FTIR对CREC同源性的区分能力具有较高准确性,且操作简便快速,具有良好的临床推广前景。

Objective:Evaluate the application of Fourier transform infrared spectroscopy in the identification of homology of carbapenem-resistant Escherichia coli(CREC).Methods:A total of 26 carbapenem-resistant Escherichia coli strains were isolated from 9 provinces in China in 2018.The 900-1200 cm^(-1) was selected as a spectral region for the Euclidean distance calculating and average linkage clustering between all isolates.The single nucleotide polymorphism(SNP)was analyzed by whole genome sequencing(WGS).Results:Twenty-six CREC strains were divided into 14 infrared spectros copy(IR)types by FTIR.The same IR type belonged to the same sequence type type.Compared with cluster analysis based on WGS,the consistency of FTIR cluster analysis was 92.3%(24/26).Conclusions:FTIR presented excellent performance in identification of homology of CREC.Besides,with the advantages of simple operation and rapid acquisition of results,FTIR may be a useful tool in clinical labs.