滩羊脂肪和肥胖相关基因(FTO)多态性及其与部分屠宰指标相关性研究

Study on fat mass and obesity associated gene (FTO) polymorphism and their correlation with some slaughter indexes in Tan sheep

为了研究滩羊脂肪和肥胖相关基因(FTO)SNP位点多态性及其与部分屠宰性状间的相关性,试验选择108只健康的成年滩羊,采集其抗凝血提取基因组DNA,利用PCR和DNA测序的方法对FTO基因中rs4784351、rs7203051两个SNP位点进行多态性检测,并分析其与滩羊屠宰指标(活体重量、胴体重量、肾脏脂肪重量、胃肠脂肪重量、尾部脂肪重量)间的相关性。结果表明:rs4784351位点在108只滩羊中共检测到3种基因型(TT型、TC型和CC型),由2个等位基因(T、C)控制,T为优势等位基因,该位点分别与滩羊胴体重量和尾部脂肪重量呈显著和极显著相关(P<0.05,P<0.01)。而rs7203051位点只有19只滩羊检测出了多态性,因此不做统计分析。说明FTO基因的rs4784351位点与滩羊尾部脂肪重量有显著的相关性,可以作为滩羊育种选择的重要参考分子标记位点。

In order to study fat mass and obesity-related gene(FTO) SNP polymorphism and their correlation with some slaughter traits in Tan sheep, 108 healthy adult Tan sheep were selected, and its anticoagulant were collected and genomic DNA was extracted. The polymorphism of FTO gene SNP locus(rs4784351 and rs7203051) were detected by using the method of PCR and DNA sequencing, and the correlation between the SNP loci and the slaughter indicators(living weight, carcass weight, kidney fat weight, gastrointestinal fat weight, tail fat weight) of Tan sheep was analyzed. The results showed that three genotypes(TT, TC and CC) were detected in 108 samples at rs4784351 locus, which were controlled by two alleles(T and C),and T was the dominant allele.The rs4784351 locus was significantly and extremely significantly correlated with carcass weight and tail fat weight of Tan sheep(P<0.05,P<0.01). The rs7203051 locus polymorphism was only detected in 19 Tan sheep, so no statistical analysis was performed. These results indicated that rs4784351 locus of FTO gene was significantly correlated with tail fat weight of Tan sheep, which could be used as an important reference molecular marker site for breeding selection of Tan sheep.