超声波、NaCl胁迫及协同处理对苦荞麦萌发、生理变化及抗氧化性的影响

Effects of Ultrasonic Wave,NaCl Stress and Synergistic Treatment on Germination,Physiological Changes and Antioxidant Activity of Tartary Buckwheat

采用不同超声波功率、超声时间、不同浓度NaCl溶液及超声波协同NaCl溶液对苦荞麦种子进行预处理,研究上述处理对苦荞麦萌发及生理变化等的影响。三种处理条件下苦荞萌发率平均达75.33%。超声波协同NaCl(U320 W/30 min+NaCl 5.0 mmol/L)处理的芽苗总酚含量最高,其含量为2.89±0.01 mg/g,该处理下MDA含量最低,为5.62±0.02 mmol/g。超声波协同NaCl处理(U320 W/20 min+NaCl 5.0 mmol/L)芽苗总黄酮含量最高,达1.71±0.03 g/100 g,该处理下芽苗的POD活性最高,达61.78±1.68 U·g^(-1)·min^(-1)。超声波处理(320 W/30 min)SOD活性最高达61.90 U/mg,比对照组增加0.34倍。NaCl溶液处理(2.5 mmol/L)还原糖含量最高,达到12.85±0.28 mg/g。超声波处理(280 W/30 min)DPPH清除能力最高,达92.93±1.58%。结果表明,适宜功率的超声波和低浓度的NaCl胁迫及协同处理均能在一定程度上促进苦荞麦种子萌发,且有利于提高其生理变化及抗氧化性。

Different ultrasonic power,ultrasonic time,different concentration of NaCl solution and ultrasonic combined with NaCl solution were used to pretreat Tartary buckwheat seeds.The effects of the above treatments on the germination and physiological changes of Tartary buckwheat seeds were studied.The results showed that the average germination rate of Tartary buckwheat reached 75.33%under the three treatments.The highest content of total phenol was found in the shoots treated with ultrasonic combined with NaCl(U320 W/30 min+NaCl 5.0 mmol/L),and the lowest content of MDA was 5.62±0.02 nmol/g.The content of total flavonoids was the highest in the shoots treated with ultrasound and NaCl(U320 W/20 min+NaCl 5.0 mmol/L),and the POD activity was the highest in the shoots treated with ultrasound and NaCl(U320 W/20 min+NaCl 5.0 mmol/L),which was 61.78±1.68 U·g^(-1)·min^(-1).The SOD activity of ultrasonic treatment(320 W/30 min)was up to 61.90 U/mg,0.34 times higher than that of CK.The highest reducing sugar content was 12.85±0.28 mg/g in 2.5 mmol/L NaCl solution treatment.Ultrasonic treatment(280 W/30 min)had the highest DPPH scavenging ability,up to 92.93±1.58%.The results showed that the appropriate power of ultrasonic and low concentration of NaCl stress and the synergical treatment could promote the germination of Tagopyrum buckwheat seeds to a certain extent,and was beneficial to improve its physiological changes and antioxidant activity.