牦牛蹄筋胶原蛋白提取工艺优化

Optimization of Extraction Process of Collagen from Yak Tendon

为高效制备牦牛蹄筋胶原蛋白,以牦牛蹄筋为原料,采用热水法和酶解法提取牦牛蹄筋胶原蛋白,选择合适的提取方法,通过单因素试验分析料液比、温度、时间、加酶量对牦牛蹄筋胶原蛋白得率、提取率及纯度的影响,以正交试验优化酶解条件。结果表明:酶解法提取牦牛蹄筋胶原蛋白效果较好,最佳提取条件为料液比1∶12(g/mL)、加酶量2.5%、温度43℃、时间11 h,胶原蛋白的得率、提取率及纯度分别为14.91%,69.94%和89.44%。

In order to efficiently extract the collagen from yak tendon,the yak tendon was used as raw material to extract the collagen by hot water and enzymolysis method,and the best extraction method was selected.On the basis of the optimal extraction method,the effects of different ratios of solid-to-liquid,temperature,time and enzyme dosage on the yield,extraction rate and purity of yak tendon collagen were studied by single factor ex-periment.The extraction conditions were optimized by orthogonal experiment.The results showed that enzymol-ysis method had better effect on yak tendon collagen extraction.The optimal extractionconditionswere deter-mineas follows:solid-to-liquid ratio 1∶12(g/mL),and enzyme dosage 2.5%,and extraction at 43℃for 11 h.Under these conditions,the yield,extraction rate and purity of collagen were 14.91%,69.94%and 89.44%,re-spectively.