摘要
The multifunctional protein p100 is a vital transcriptional regulator that increases gene transcription by forming a physical bridge between promoter-specific transcription factors and the basal transcription machinery.To identify potential signal transduction pathways in which human p100 acts as a coregulator and to find target promoter regions that may interact with p100,we performed a promoter microarray assay called chromatin immunoprecipitation-guided ligation and selection(ChIP-GLAS).From this assay,we determined that a set of promoter fragments,including several factors in the transforming growth factor beta(TGF-β)signaling pathway,exhibited interaction with p100.The ChIP-GLAS data were validated by RT-PCR assessing the mRNA expression of various factors in the TGF-b signaling pathway in cell lines.
基金
This work was supported by grants from the National Basic Research Program(973 Program,2009CB918903)
863 Project of the Ministry of Science and Technology of China(2007AA02Z115)
NSFC(90919032,30970562,30670441,30811130394 and 30870562)
Tianjin Municipal Science and Technology Commission(08ZCGHHZ01900 and 08JCYBJC07700)
Specialized Fund for the Doctoral Program of Higher Education(20091202110001)
Tianjin Educational Committee Foundation(2008ZD01).