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微小RNA-146a及白细胞介素-1受体相关激酶在脑膜瘤组织的表达及其临床意义 被引量:1

Expression and cinical significance of microRNA-146a and interleukin-1 receptor associated kinases in meningioma
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摘要 目的:探讨微小RNA(miR)-146a及白细胞介素-1受体相关激酶(IRAK1)在脑膜瘤组织中的表达及其与脑膜瘤临床特征的相关性。方法:选取50例脑膜瘤组织与10例因去骨瓣减压术获得的硬脑膜组织作为正常对照组,实时定量反转录聚合酶链反应(RT-qPCR)检测各组组织中miR-146a的表达水平及IRAK1 mRNA的相对表达水平;免疫组织化学方法检测IRAK1蛋白的表达水平;分析两者表达的相关性及其与脑膜瘤患者临床特征之间的关系。结果:脑膜瘤组织中miR-146a mRNA的表达水平明显低于正常脑膜组,IRAK1的表达水平明显高于正常脑膜组,且两者间的表达呈负相关(r=-0.305),差异均有统计学意义(t=-3.896、4.468,P值均<0.05)。WHOⅡ级、Ⅲ级的脑膜瘤组织中,miR-146a mRNA的表达水平均低于WHOⅠ级组(t=4.728、8.227),IRAK1 mRNA的表达水平均高于WHOⅠ级组(t=-4.262,t=-4.287),差异有统计学意义(P<0.05)。而WHOⅡ级与Ⅲ级间差异无统计学意义(P>0.05)。IRAK1蛋白的表达随着脑膜瘤组织各病理级别间差异明显。结论:miR-146a可能为脑膜瘤的抑癌基因,负调控IRAK1的表达。 Objective To explore the expression of microRNA(miR)-146a and interleukin-1 receptor associated kinases(IRAK1)in meningioma and their correlation with the clinical features of meningioma.Methods Totally,50 cases of meningioma tissues and 10 cases of dural tissues obtained after decompressive craniectomy were studied.MiR-146a and IRAK1 mRNA expression levels were detected by RT-PCR from different groups.The expression of IRAK1 protein was analyzed by immunohistochemistry.Pearson correlation analyses were performed to explore associations between miR-146a and IRAK1 mRNA expression.Also,the relationships between these changes with clinical data of the meningioma patients were analyzed.Results The expression level of miR-146a mRNA in meningioma tissue was significantly lower than that of the normal meningeal group,and the expression level of IRAK1 was significantly higher than that of the normal dura mater group,and the relationship between the two was negatively correlated(r=-0.305),the differences is statistically significant(t=-3.896,4.468,P<0.05).In WHO gradeⅡand gradeⅢmeningioma,the expression level of miR-146a mRNA was lower than the WHO gradeⅠgroup(t=4.728,8.227),and the expression level of IRAK1 mRNA was higher than the WHO gradeⅠgroup(t=-4.262,-4.287),the difference was statistically significant(P<0.05).But there was no statistically significant difference between WHO gradeⅡandⅢmeningioma(P>0.05).The expression of IRAK1 protein varies significantly with the pathological grades of meningioma(χ^(2)=20.044,P<0.05).Conclusion MiR-146a is a tumor suppressor gene for meningioma,and negatively regulates the expression of IRAK1.
作者 陈卫峰 吴倩岚 杨亮 吴震 陈镭 赵春梅 赵宗茂 Chen Weifeng;Wu Qianlan;Yang Liang;Wu Zhen;Chen Lei;Zhao Chunmei;Zhao Zongmao(Department of Neurosurgery,the Second Hospital of Hebei Medical University,Shijiazhuang 050000,China;Department of Neurosurgery,Handan Central Hospital,Handan 056000,China;Department of Pathology,the First Hospital of Handan,Handan 056000,China;Department of Neurosurgery,Beijing Tiantan Hospital,Capital Medical University,Beijing 100070,China;Department of Neurosurgery,Tianjin Fifth Central Hospital,Tianjin 300450,China)
出处 《中华实验外科杂志》 CAS 北大核心 2021年第7期1349-1352,共4页 Chinese Journal of Experimental Surgery
基金 国家自然科学基金(81870984) 科技部国家重点研发计划项目-政府间国际科技创新合作重点专项(2017YFEO110400) 河北省自然科学基金-京津冀基础研究合作专项项目(H2018206675) 河北省国际科技合作基地建设专项(193977143D) 2017年河北省政府资助临床医学优秀人才培养和基础课题研究项目 2019年河北省政府资助临床医学优秀人才培养项目 2021年河北省医学科学研究课题(20210973)。
关键词 脑膜瘤 微小RNA 临床特征 Meningioma MicroRNA-146a Clinical characteristics
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  • 1Borel F,Konstantinova P,Jansen PL. Diagnostic and therapeutic potential of miRNA signatures in patients with hepatocellular carcinoma[J].Journal of Hepatology,2012.1371-1383.
  • 2Braconi C,Henry JC,Kognre T. The role of microRNAs in human liver cancers[J].Seminars in Oncology,2011.752-763.
  • 3Gui J,Tian Y,Wen X. Serum microRNA characterization identifies miR-885-5p as a potential marker for detecting liver pathologies[J].Clinical Science(London),2011.183-193.
  • 4Akkiz H,Bayram S,Bekar A. No association of pre-microRNA-146a rs2910t64 polymorphism and risk of hepatocellular carcinoma development in Turkish population:a case-control study[J].Gene,2011.104-109.
  • 5Xu T,Zhu Y,Wei QK. A functional polymorphism in the miR146a gene is associated with the risk for hepatocellular carcinoma[J].Carcinogenesis,2008.2126-2131.
  • 6Xiang Y,Fan S,Cao J. Association of the microRNA-499 variants with susceptibility to hepatocellular carcinoma in a Chinese population[J].Molecular Biology Reports,2012.7019-7023.
  • 7Chen G,Kronenberger P,Teugels E. Influence of RT-qPCR primer position on EGFR interference efficacy in lung cancer cells[J].Biological Procedures Online,2011.1.
  • 8Chen G,Kronenberger P,Umelo IA. Quantification of epidermal growth factor receptor T790M mutant transcripts in lung cancer cells by real-time reverse transcriptase-quantitative polymeerse chain reaction[J].Analytical Biochemistry,2010.266-268.
  • 9Livak K J,Schmittgen TD. Analysis of relative gene expression data using,real-time quantitative PCR and the 2[-Delta Delta C (T)]Method[J].Methods:A Companion to Methods in Enzymology,2001.402-408.
  • 10Kogo R,Mimori K,Tanaka F. Clinical significance of miR-146a in gastric cancer cases[J].Clinical Cancer Research,2011.4277-4284.

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